The CNGCb and CNGCd genes from Physcomitrella patens moss encode for thermosensory calcium channels responding to fluidity changes in the plasma membrane.
The CNGCb and CNGCd genes from Physcomitrella patens moss encode for thermosensory calcium channels responding to fluidity changes in the plasma membrane.
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DOI：
10.1007/s12192-013-0436-9
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发表时间：
2014-01
影响因子：
3.8
通讯作者：
Goloubinoff, Pierre
中科院分区：
文献类型：
--
作者：
Finka, Andrija;Goloubinoff, Pierre
关键词：
陆地植物需要精确的温度传感器来及时建立分子防御，以应对即将到来的有害热浪。质膜嵌入的环核苷酸门控Ca 2+通道（CNGC）可以将膜流动性的轻微变化转化为有效的热休克反应，导致热休克蛋白（HSP）的积累，从而防止不稳定蛋白和膜的热损伤。在这里，我们删除了CNGCd基因的靶向诱变在两个小立碗藓转基因藓系含有热诱导型HSP-GUS报告盒或组成型UBI-Aequorin盒。稳定的CNGCd基因敲除突变引起超温敏苔藓表型，其中热诱导的质外体Ca 2+和GUS的胞质积累的进入被触发在较低的温度比野生型。人工膜流化器和升高的温度的综合作用表明，CNGCd和CNGCb的基因产物是作为一个敏感的蛋白脂质热电偶的钙通道的旁系同源亚基。取决于温度升高的速率、热启动先决条件的持续时间和强度，陆生植物可能因此获得一系列基于HSP的耐热性机制，以对抗即将到来的、否则致命的极端热浪。本文的在线版本（doi：10.1007/s12192-013-0436-9）包含补充材料，可供授权用户使用。
Land plants need precise thermosensors to timely establish molecular defenses in anticipation of upcoming noxious heat waves. The plasma membrane-embedded cyclic nucleotide-gated Ca2+ channels (CNGCs) can translate mild variations of membrane fluidity into an effective heat shock response, leading to the accumulation of heat shock proteins (HSP) that prevent heat damages in labile proteins and membranes. Here, we deleted by targeted mutagenesis the CNGCd gene in two Physcomitrella patens transgenic moss lines containing either the heat-inducible HSP-GUS reporter cassette or the constitutive UBI-Aequorin cassette. The stable CNGCd knockout mutation caused a hyper-thermosensitive moss phenotype, in which the heat-induced entry of apoplastic Ca2+ and the cytosolic accumulation of GUS were triggered at lower temperatures than in wild type. The combined effects of an artificial membrane fluidizer and elevated temperatures suggested that the gene products of CNGCd and CNGCb are paralogous subunits of Ca2+channels acting as a sensitive proteolipid thermocouple. Depending on the rate of temperature increase, the duration and intensity of the heat priming preconditions, terrestrial plants may thus acquire an array of HSP-based thermotolerance mechanisms against upcoming, otherwise lethal, extreme heat waves. The online version of this article (doi:10.1007/s12192-013-0436-9) contains supplementary material, which is available to authorized users.
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DOI：
10.1073/pnas.95.7.3513
发表时间：
1998-03-31
影响因子：
11.1
作者：
Horváth, I;Glatz, A;Vígh, L
通讯作者：
Vígh, L
影响因子：
7.2
作者：
Mishkind, Michael;Vermeer, Joop E. M.;Munnik, Teun
通讯作者：
Munnik, Teun
影响因子：
3.8
作者：
de Marco, A;Vigh, L;Goloubinoff, P
通讯作者：
Goloubinoff, P
影响因子：
4.9
作者：
Fischer C;Kugler A;Hoth S;Dietrich P
通讯作者：
Dietrich P
影响因子：
16
作者：
Petesch, Steven J.;Lis, John T.
通讯作者：
Lis, John T.