A paramagnetic CEST agent for imaging glucose by MRI
A paramagnetic CEST agent for imaging glucose by MRI
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DOI：
10.1021/ja038345f
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发表时间：
2003-12-17
影响因子：
15
通讯作者：
Sherry, AD
中科院分区：
文献类型：
--
作者：
Zhang, SR;Trokowski, R;Sherry, AD
DOTA−四酰胺配体的铕(III)络合物（DOTA = 1,4,7,10-四氮杂环十二烷-N,N',N' ',N' ''-四乙酸）含有两个硼酸苯酯悬臂，在 pH 7 下以 383 M-1 的缔合常数可逆地结合葡萄糖。葡萄糖结合导致单个 Eu(III) 结合之间的水交换减慢水分子和大量水，这可以通过 MRI 使用化学交换饱和转移 (CEST) 成像序列进行成像。这种代谢物响应性顺磁性 CEST 试剂对生理重要范围 (0−20 mM) 内的葡萄糖变化做出响应，因此它提供了使用大量水质子作为天线对组织中葡萄糖进行高灵敏度 MR 成像的可能性。
The europium(III) complex of a DOTA−tetraamide ligand (DOTA = 1,4,7,10-tetraazacyclododecane-N,N‘,N‘ ‘,N‘ ‘‘-tetraacetic acids) containing two phenyl boronate pendent arms binds glucose reversibly with an association constant of 383 M-1at pH 7. Glucose binding results in slowing of water exchange between a single Eu(III)-bound water molecule and bulk water, and this can be imaged by MRI using chemical exchange saturation transfer (CEST) imaging sequence. This metabolite-responsive paramagnetic CEST agent responds to changes in glucose over the physiologically important range (0−20 mM), and thus it offers the possibility of high-sensitivity MR imaging glucose in tissues using bulk water protons as antenna.