Analysis of abscisic acid responsive proteins in Brassica napus guard cells by multiplexed isobaric tagging.
Analysis of abscisic acid responsive proteins in Brassica napus guard cells by multiplexed isobaric tagging.
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DOI：
10.1016/j.jprot.2009.11.002
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发表时间：
2010-02
影响因子：
3.3
通讯作者：
Mengmeng Zhu;B. Simons;Ning Zhu;D. Oppenheimer;Sixue Chen
中科院分区：
文献类型：
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作者：
Mengmeng Zhu;B. Simons;Ning Zhu;D. Oppenheimer;Sixue Chen
保卫细胞在叶表皮上形成气孔，在植物气体交换和防御病原体方面发挥着重要作用。脱落酸 (ABA) 是一种植物激素，可由干旱诱导并导致气孔关闭。保卫细胞一直是研究 ABA 信号转导的首要模型系统。尽管在 ABA 信号通路分子成分的鉴定方面取得了重大进展，但我们对蛋白质成分的了解非常有限。在这里，我们采用最近开发的多重同量异位标记技术来识别甘蓝型油菜保卫细胞中的 ABA 响应蛋白。在对照和 ABA 处理的样品中，通过相对定量信息鉴定出总共 431 种独特的蛋白质。参与应激和防御的蛋白质构成了丰度增加的 66 种蛋白质中的主要类别。 38 种蛋白质的丰度下降，并分为几个功能组，包括代谢和蛋白质合成。许多蛋白质尚未被报道为 ABA 响应或参与气孔运动。大部分蛋白质编码基因含有 ABA 响应元件。这项研究不仅建立了 ABA 响应蛋白的全面清单，而且还鉴定了新的蛋白，以进一步研究它们在保卫细胞 ABA 信号传导中的功能。
Guard cells, which form stomata on the leaf epidermis, play important roles in plant gas exchange and defense against pathogens. Abscisic acid (ABA) is a phytohormone that can be induced by drought and leads to stomatal closure. Guard cells have been a premier model system for studying ABA signal transduction. Despite significant progress on the identification of molecular components in the ABA signaling pathway, our knowledge of the protein components is very limited. Here, we employ a recently developed multiplexed isobaric tagging technology to identify ABA-responsive proteins in Brassica napus guard cells. A total of 431 unique proteins were identified with relative quantitative information in control and ABA-treated samples. Proteins involved in stress and defense constituted a major group among the 66 proteins with increased abundance. Thirty-eight proteins were decreased in abundance and fell into several functional groups including metabolism and protein synthesis. Many of the proteins have not been reported as being ABA responsive or involved in stomatal movement. A large percentage of the protein-coding genes contained ABA-responsive elements. This study not only established a comprehensive inventory of ABA-responsive proteins, but also identified new proteins for further investigation of their functions in guard cell ABA signaling.