Cell cycle-dependent binding of yeast heat shock factor to nucleosomes.
Cell cycle-dependent binding of yeast heat shock factor to nucleosomes.
复制标题
酵母热休克因子与核小体的细胞周期依赖性结合。
DOI：
10.1128/mcb.20.17.6435-6448.2000
复制
发表时间：
2000
影响因子：
5.3
通讯作者：
Gross,DS
中科院分区：
文献类型：
--
作者：
Venturi,CB;Erkine,AM;Gross,DS
In the nucleus, transcription factors must contend with the presence of chromatin in order to gain access to their cognate regulatory sequences. As most nuclear DNA is assembled into nucleosomes, activators must either invade a stable, preassembled nucleosome or preempt the formation of nucleosomes on newly replicated DNA, which is transiently free of histones. We have investigated the mechanism by which heat shock factor (HSF) binds to target nucleosomal heat shock elements (HSEs), using as our model a dinucleosomal heat shock promoter (hsp82-ΔHSE1). We find that activated HSF cannot bind a stable, sequence-positioned nucleosome in G1-arrested cells. It can do so readily, however, following release from G1arrest or after the imposition of either an early S- or late G2-phase arrest. Surprisingly, despite the S-phase requirement, HSF nucleosomal binding activity is restored in the absence ofhsp82replication. These results contrast with the prevailing paradigm for activator-nucleosome interactions and implicate a nonreplicative, S-phase-specific event as a prerequisite for HSF binding to nucleosomal sites in vivo.
