Active tissue factor shed from human arterial smooth muscle cells adheres to artificial surfaces.
Active tissue factor shed from human arterial smooth muscle cells adheres to artificial surfaces.
复制标题
从人动脉平滑肌细胞脱落的活性组织因子粘附在人造表面上。
DOI：
10.1163/156856200744282
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Turitto,VT
中科院分区：
文献类型：
--
作者：
Hathcock,JJ;Hall,CL;Turitto,VT
本研究通过一系列的体外实验，阐明了一种流动介导的过程，通过该过程，凝血的主要启动剂活性组织因子（TF）可从血管的质膜转移， 平滑肌细胞（VSMCs）与人造表面（例如通常与血管内植入物相关的那些）的细胞。对静止和激活的大鼠VSMC的研究表明， 高剪切应力（τw= 250 dyn cm-2）导致TF活性从细胞表面丧失。随后对人VSMCs的实验表明，VSMCs持续释放 活性TF进入其细胞外介质，可能是以脂质囊泡或微粒的形式，流体剪切应力（τw= 50 dyn cm-2）或化学激动剂（A23187） 可以大大加速这种释放。用各种聚合物和金属材料进行的实验表明，从VSMCs脱落的TF能够粘附在这些表面并促进活化 凝血因子X（FX）在材料表面。在广泛的血流动力学切应力（0-20 dyn cm-2）下，细胞外TF与未涂层和人血浆涂层表面强烈结合。 当细胞外，VSMC衍生的TF混合物灌注在Ti 6-4表面上时，发现TF的粘附是时间依赖性的，随着时间的推移逐渐积累在材料表面上。一个重要的 血管内装置的设计或成功的标准可能与它们与从细胞表面脱落的TF相互作用的能力有关。这一点尤其重要，因为TF可能导致血栓性并发症， 其产物也可增加细胞增殖。
Through a series of in vitro assays, this study outlines a flow-mediated process by which active tissue factor (TF), the prime initiator of coagulation, may be transferred from the plasma membrane of vascular smooth muscle cells (VSMCs) to that of artificial surfaces such as those typically associated with intravascular implants. Studies with quiescent and activated rat VSMCs demonstrated that pathologically high shear stresses (τw= 250 dyn cm-2) resulted in the loss of TF activity from the cell surface. Subsequent experiments with human VSMCs showed that VSMCs continuously release active TF into their extracellular medium, presumably in the form of lipid vesicles or microparticles, and that fluid shear stress (τw= 50 dyn cm-2) or chemical agonists (A23187) can significantly accelerate this release. Experiments with a wide array of polymeric and metallic materials showed that the TF shed from VSMCs was able to adhere to these surfaces and promote the activation of coagulation factor X (FX) at the material surface. Extracellular TF bound strongly to both uncoated and human plasma coated surfaces under a wide range of hemodynamic shear stresses (0-20 dyn cm-2). When an extracellular, VSMC-derived TF mixture was perfused over Ti 6-4 surfaces, the adhesion of TF was found to be time-dependent, gradually accumulating on the material surface over time. Thus an important criterion in the design or success of intravascular devices may be related to their ability to interact with TF, shed from cell surfaces. This is especially important as TF may lead to thrombotic complications, the products of which may also increase cellular proliferation.