EVIDENCE FOR A FUNCTIONAL MEMBRANE BARRIER IN THE TRANSITION ZONE BETWEEN THE FLAGELLUM AND CELL BODY OF CHLAMYDOMONAS-EUGAMETOS GAMETES
EVIDENCE FOR A FUNCTIONAL MEMBRANE BARRIER IN THE TRANSITION ZONE BETWEEN THE FLAGELLUM AND CELL BODY OF CHLAMYDOMONAS-EUGAMETOS GAMETES
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DOI：
10.1007/bf00391231
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发表时间：
1986-01-01
期刊：
影响因子：
4.3
通讯作者：
VANDENENDE, H
中科院分区：
文献类型：
--
作者：
MUSGRAVE, A;DEWILDT, P;VANDENENDE, H
证据支持的概念，一个功能性的膜屏障的过渡区在每个鞭毛的Chlamyeliumeugametos配子的基础。这使得存在于细胞体表面的凝集因子不可能扩散或被转运到鞭毛膜。证据如下：（1）鞭毛膜的糖蛋白组成与胞体质膜的糖蛋白组成有很大的不同。(2)用放线菌酮、衣霉素或α，α。- 联吡啶变得不可凝集，但细胞体上凝集因子的来源不受影响。(3)即使在自然条件下，当鞭毛是不可凝集的，例如是面对面对或在黑暗中不可凝集的适当细胞株中，细胞体也保持活性凝集素的正常补体。(4)当活细胞的鞭毛用与荧光素结合的抗体标记时，标记物不会扩散到细胞体表面。(5)当配子融合形成面对面对时，每个细胞体的原始交配型特异性抗原性缓慢丧失（可能是由于抗原扩散到两个细胞体上），而鞭毛表面的特异性抗原性得以保持。即使当面对面对的鞭毛从具有混合抗原性的细胞体再生时，鞭毛的抗原性仍然是交配型特异性的。(6)证据是存在的一个池内的细胞体，但不是在细胞的外表面上的凝集因子。
Evidence is presented which supports the concept of a functional membrane barrier in the transition zone at the base of each flagellum of Chlamydomonas eugametos gametes. This makes it unlikely that agglutination factors present on the surface of the cell body can diffuse or be transported to the flagellar membrane. The evidence is as follows: (1) The glycoprotein composition of the flagellar membrane is very different to that of the cell-body plasma membrane. (2) The flagella of gametes treated with cycloheximide, tunicamycin or .alpha., .alpha.''-dipyridyl become non-agglutinable but the source of agglutination factors on the cell body is not affected. (3) Even under natural conditions when the flagella are non-agglutinable, for example is vis-a-vis pairs or in appropriate cell strains that are non-agglutinable in the dark, the cell bodies maintain the normal complement of active agglutinins. (4) When flagella of living cells are labeled with antibodies bound to fluorescein, the label does not diffuse onto the cell-body surface. (5) When gametes fuse to form vis-a-vis pairs, the original mating-type-specific antigenicity of each cell body is slowly lost (probably due to the antigens diffusing over both cell bodies), while the specific antigenicity of the flagellar surface is maintained. Even when the flagella of vis-a-vis pairs are regenerated from cell bodies with mixed antigenicity, the antigenicity of the flagella remains mating-type-specific. (6) Evidence is presented for the existence of a pool of agglutination factors within the cell bodies but not on the outer surface of the cells.