piggyBac-mediated germline transformation of the malaria mosquito Anopheles sinensis (Diptera: Culicidae)
piggyBac-mediated germline transformation of the malaria mosquito Anopheles sinensis (Diptera: Culicidae)
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PiggyBac 介导的疟疾蚊子中华按蚊（双翅目：蚊科）种系转化
DOI：
10.1111/1744-7917.12836
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发表时间：
2020
期刊：
影响因子：
4
通讯作者：
He Zheng-Bo
中科院分区：
文献类型：
--
作者：
Liu Jin-Gang;Qiao Liang;Zhang Jia-Jun;Chen Bin;He Zheng-Bo
编辑先生，中华按蚊是我国间日疟的主要传播媒介。鉴于安所带来的风险。在中华按蚊中，已经使用了许多方法来控制这种媒介，最常见的是化学预防。然而，杀虫剂的广泛使用使蚊子受到强烈的选择压力，导致抗性的发展、蚊子行为的变化和疟疾媒介物种的转变（Zhang et al.，2017年; He等人，2019年）。因此，迫切需要新的以蚊子为中心的控制或消除疟疾传播的策略。最近基于按蚊媒介种群修饰的媒介控制策略，如基于遗传学的不育昆虫技术和种群替代技术，在根除疟疾方面显示出巨大的前景（J. P.，2016; Carballar-Lejarazú & James，2017）。遗传转化技术的发展极大地增加了用于改进基于遗传学的控制方法的可用选择（O 'Brochta & Jumeir，2008）。piggyBac转座因子的遗传转化最初发现于尺蠖Trichoplusia ni中，最近在一系列昆虫物种中发现，包括双翅目，鳞翅目，膜翅目和鞘翅目的成员（PiggyBac，2002; Adolfi & Lycett，2018）。然而，转基因技术在An.中国还没有
Dear Editor, Anopheles sinensis is the primary vector of vivax malaria in China. Given the risks posed by An. sinensis, many methods have been used to control this vector, most commonly chemical prevention. However, the extensive use of insecticides has subjected mosquitoes to intense selection pressure, resulting in the development of resistance, variations in mosquito behavior, and a shift in malarial vector species (Zhang et al., 2017; He et al., 2019). Therefore, new mosquito-centered strategies for controlling or eliminating malaria transmission are urgently required. Recent vector control strategies based on population modification of Anopheles vectors, such as the genetics-based sterile insect technique and population-replacement techniques, have shown great prospects in malaria eradication (Handler, 2016; Carballar-Lejarazú & James, 2017). The development of genetic transformation technologies has greatly increased the available options for improving thevvse genetics-based control methods (O’Brochta & Handler, 2008). Genetic transformation of piggyBac transposable elements, which were originally discovered in the looper moth Trichoplusia ni, has recently been discovered in a range of insect species, including members of Diptera, Lepidoptera, Hymenoptera, and Coleoptera (Handler, 2002; Adolfi & Lycett, 2018). However, the use of transgenic techniques in An. sinensis has not yet