cDNAs for canavalin and concanavalin A from Canavalia gladiata seeds. Nucleotide sequence of cDNA for canavalin and RNA blot analysis of canavalin and concanavalin A mRNAs in developing seeds.
cDNAs for canavalin and concanavalin A from Canavalia gladiata seeds. Nucleotide sequence of cDNA for canavalin and RNA blot analysis of canavalin and concanavalin A mRNAs in developing seeds.
复制标题
来自刀豆种子的刀豆球蛋白和刀豆球蛋白 A 的 cDNA。
DOI：
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发表时间：
1988
期刊：
影响因子：
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通讯作者：
T. Minamikawa
中科院分区：
文献类型：
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作者：
D. Yamauchi;K. Nakamura;T. Asahi;T. Minamikawa
采用大肠杆菌表达载体引发的cDNA合成方法，从刀豆幼胚中提取富含poly（A）的总RNA，构建了刀豆cDNA表达文库。基本上全长cDNA克隆的两个种子蛋白，刀豆球蛋白和伴刀豆球蛋白A，从库中选择的免疫筛选的殖民地和体外RNA合成和翻译。从相应cDNA的核苷酸序列中确定了刀豆球蛋白的完整氨基酸序列，并发现其与其他豆类的7S种子蛋白非常同源。cDNA的核苷酸序列预测成熟刀豆球蛋白的氨基末端的前体中有26个氨基酸的延伸。通过RNA印迹杂交估计了种子发育的连续阶段的刀豆球蛋白mRNA和伴刀豆球蛋白A mRNA水平，结果表明这两种mRNA水平受到不同的调节。
By a method of Escherichia coli expression-vector-primed cDNA synthesis, a cDNA expression library was constructed from total poly(A)-rich RNA that was prepared from immature embryos of Canavalia gladiata. Essentially full-length cDNA clones for two seed proteins, canavalin and concanavalin A, were selected from the library by immunological screening of the colonies and in vitro RNA synthesis and translation. The complete amino acid sequence of canavalin was determined from the nucleotide sequence of the corresponding cDNA and was found to be very homologous to 7S seed proteins of other legumes. The nucleotide sequence of the cDNA predicts a 26-amino-acid extension in the precursor at the amino terminus of the mature canavalin. Canavalin mRNA and concanavalin A mRNA levels at successive stages of the seed development were estimated by RNA blot hybridization and results indicated that the two mRNA levels are differently regulated.