Insulin-like growth factor-1 reverses diabetes-induced wound healing impairment in rats
Insulin-like growth factor-1 reverses diabetes-induced wound healing impairment in rats
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DOI：
10.1055/s-2007-979060
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发表时间：
1997-08-01
影响因子：
2.2
通讯作者：
Bitar, MS
中科院分区：
文献类型：
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作者：
Bitar, MS
伤口愈合不良是糖尿病的一个神秘和衰弱的并发症。关于这种疾病的发病机制尚未达成共识。最近的概念表明，IGF-1是愈合过程的重要调节剂。这种生长因子的水平在糖尿病患者的伤口环境中降低。我们测试了IGF-1给药可以预防或改善链脲佐菌素（STZ，55 mg/kg，iv）糖尿病大鼠伤口愈合障碍的前提。通过微型渗透泵将IGF-1（15 μ g/天）或安慰剂输注到标准化不锈钢背创伤室中。伤口相关参数，包括蛋白质，DNA，羟脯氨酸和巨噬细胞作为糖尿病的函数降低。用IGF-1治疗14天逆转了糖尿病效应，并使总羟脯氨酸、DNA、蛋白质和巨噬细胞数量分别增加了48%、52%、31%和40%，高于溶剂对照值。数据支持以下前提：糖尿病、伤口环境内IGF-1和/或巨噬细胞功能的相关抑制至少部分地导致这种疾病状态下的伤口愈合受损。
Impaired wound healing is an enigmatic and debilitating complication of diabetes. A consensus as to the pathogenesis of this disorder has yet to emerge. Recent concepts suggest that IGF-1 is an important regulator of the healing process. The level of this growth factor is reduced in the wound environment of diabetics. We tested the premise that IGF-1 administration may prevent or ameliorate wound healing impairment in streptozotocin (STZ, 55 mg/kg, iv) diabetic rats. IGF-1 (15 mu g/day) or placebo was infused via mini-osmotic pumps into standardized stainless steel dorsal wound chambers. Wound-related parameters including protein, DNA, hydroxyproline and macrophages were decreased as a function of diabetes. A 14-day treatment with IGF-1 reversed the diabetes effect and increased total hydroxyproline, DNA, protein and macrophage numbers by 48%, 52%, 31% and 40% above vehicle-control values, respectively. The data support the premise that diabetes, related suppression of IGF-1 and/or macrophage function within the wound environment is responsible, at least in part, for the wound healing impairment in this disease state.