Expression of miRNA-223 and NLRP3 gene in IgA patients and intervention of traditional Chinese medicine
Expression of miRNA-223 and NLRP3 gene in IgA patients and intervention of traditional Chinese medicine
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DOI：
10.1016/j.sjbs.2020.04.034
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发表时间：
2020-06-01
影响因子：
4.4
通讯作者：
Li, Liya
中科院分区：
文献类型：
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作者：
Li, Liqi;Gong, Zhaoyong;Li, Liya
目的：本研究旨在探讨伊加患者miRNA-223和NLRP 3的表达及中医药的干预作用，以了解伊加患者的基本病理改变、伊加患者miRNA-223和NLRP 3的表达及中医药治疗前后患者身体指标的变化。方法：首先，根据临床资料，将伊加肾病患者根据其病理改变分为不同的组。然后进行免疫肾的化学切片和染色步骤。免疫组化pv-9000二步法染色，检测肾脏组织中miRNA-223和NLRP 3基因的表达。然后用图像分析法进行半定量实验。结果：miRNA-223和NLRP 3基因主要表达于肾小管上皮细胞胞浆和肾组织单核细胞胞浆，在单纯蛋白尿、单纯血尿、单纯蛋白尿中的表达水平差异有统计学意义。在伊加肾病中，miRNA-223和NLRP 3的表达与24 h尿蛋白定量呈正相关。结论：本研究可为伊加肾病的临床诊断和治疗提供一定的方向和指导。(C)2020作者（S）由Elsevier B. V.代表沙特国王大学出版。
Objective: The purpose of this study was to investigate the expression of miRNA-223 and NLRP3 in IgA patients and the intervention of traditional Chinese medicine (TCM), so as to realize the basic pathological changes of IgA patients, the expression of miRNA-223 and NLRP3 in IgA patients and the changes of patients' body indexes before and after the treatment of TCM.Methods: Firstly, according to the clinical data, patients with IgA nephropathy were divided into different groups according to their pathological changes. After that, the chemical sections and staining steps of the immune kidney were carried out. Immunohistochemical pv-9000 two-step method was used to stain it. By this method, miRNA-223 and NLRP3 genes in kidney were determined. After that, the image analysis method was used for semi quantitative experiment. Finally, the intervention of TCM was used to study the changes of indicators before and after treatment.Results: miRNA-223 and NLRP3 genes could be found mainly in the cytoplasm of renal tubular epithelial cells and the interstitium of monocyte in renal tissue, and there were significant differences between miRNA-223 and NLRP3 genes in the expression levels of proteinuria alone, hematuria albuminuria alone and hematuria alone. There was a positive correlation between miRNA-223 and NLRP3 expression and 24-hour urinary protein in IgA nephropathy. In addition, it also had positive correlation with MCP-1 and IL-18.Conclusion: This study could provide some direction and guidance for clinical diagnosis and treatment of IgA nephropathy. (C) 2020 The Author(s). Published by Elsevier B.V. on behalf of King Saud University.