Fetal rat intestinal absorption of horseradish peroxidase from swallowed amniotic fluid.
Fetal rat intestinal absorption of horseradish peroxidase from swallowed amniotic fluid.
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DOI：
10.1083/jcb.56.1.106
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发表时间：
1973-01
期刊：
影响因子：
--
通讯作者：
Lev R
中科院分区：
文献类型：
--
作者：
Orlic D;Lev R
注射到羊水中的辣根过氧化物酶（HRP）被大鼠胎儿吞下，并在3-6小时内到达肠腔。然后，这种大分子蛋白质通过质膜内陷形成的顶端细胞质小管系统被柱状衬里细胞吸收。从细胞质下的刷状缘HRP被运输，在空泡内，到核上区，其中一些是保留至少18小时，并到上皮间隙。然后在整个上皮基底膜和粘膜层和粘膜下层的结缔组织细胞之间发现胞外酶。最后，可以在血液和毛细淋巴管的管腔内检测到HRP，强烈表明它是从肠道运输到循环的。
Horseradish peroxidase (HRP) injected into amniotic fluid is swallowed by rat fetuses and within 3–6 h reaches the gut lumen. This macromolecular protein is then absorbed by the columnar lining cells via a system of apical cytoplasmic tubules formed by invaginations of the plasma membrane. From cytoplasm subjacent to the brush border HRP is transported, within vacuoles, to the supranuclear region, where some is retained for at least 18 h, and to interepithelial spaces. Extracellular enzyme is then found throughout the epithelial basement membrane and between connective tissue cells of the mucosal and submucosal layers Finally, HRP can be detected within lumina of blood and lymphatic capillaries, strongly suggesting that it is transported from the intestine to the circulation.