Assay of Brain Endogenous Formaldehyde With 2, 4-Dinitrophenylhydrazine Through UV-HPLC*: Assay of Brain Endogenous Formaldehyde With 2, 4-Dinitrophenylhydrazine Through UV-HPLC*
Assay of Brain Endogenous Formaldehyde With 2, 4-Dinitrophenylhydrazine Through UV-HPLC*: Assay of Brain Endogenous Formaldehyde With 2, 4-Dinitrophenylhydrazine Through UV-HPLC*
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DOI：
10.3724/sp.j.1206.2011.00407
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发表时间：
2012-01
影响因子：
0.3
通讯作者：
S. Tao;Yan-fei Wei;Rong He
中科院分区：
文献类型：
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作者：
S. Tao;Yan-fei Wei;Rong He
随着年龄的增长，内源性甲醛蓄积引起的慢性脑损伤被认为是散发性老年痴呆的危险因素之一，因此，有必要测定脑内甲醛的浓度。（2，4-DNPH）测定中，顶叶、额叶、颞叶、枕叶、海马、用10%三氯乙酸匀浆法对猪脑（n=5）小脑和脑干组织进行了紫外-高效液相色谱分析在pH =1.0的条件下，不仅避免了污染蛋白质等物质的干扰，而且消除了FA-DNPH衍生物的分步萃取（二、4-二硝基苯腙），提高方法学灵敏度用改进的方法测定猪脑组织中甲醛含量为75.5~83.4 μmol/kg新鲜脑组织，低、高浓度甲醛加标回收率为95.96%~102.04%结果表明，改良的2，4-DNPH法可用于脑组织中内源性甲醛的测定。
Chronic impairments of brain caused by accumulation of endogenous formaldehyde as ageing is thought as one of the risk factors for sporadic age-related dementia.Thus,it is necessary to determine the endogenous concentration of formaldehyde in brain.Using 2,4-dinitrophenylhydrazine(2,4-DNPH) in the assay, the concentrations of formaldehyde in parietal lobe,frontal lobe,temporal lobe,occipital lobe,hippocampus, cerebellum and brainstem of porcine brain(n=5) were measured through UV-HPLC.Homogenizing brain tissues with 10% trichloroacetic acid(pH =1.0) not only avoided the disturbance from contaminated protein and other compounds,but also eliminated the step extraction of FA-DNPH derivative(2,4-dinitrophenylhydrazone) and improve the methodological sensitivity(P 0.05).The porcine brain formaldehyde was 75.5～83.4 μmol/kg fresh brain tissues measured by this improved method.Recoveries of spiked formaldehyde at low and high level were 95.96%～102.04% with relative standard deviations less than 10%.This suggests that the modified 2,4-DNPH method is effective enough to measure the endogenous formaldehyde in brain tissues.