Model of Host-Pathogen Interaction Dynamics Links In Vivo Optical Imaging and Immune Responses.
Model of Host-Pathogen Interaction Dynamics Links In Vivo Optical Imaging and Immune Responses.
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DOI：
10.1128/iai.00606-16
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发表时间：
2017-01
影响因子：
3.1
通讯作者：
Stumpf MPH
中科院分区：
文献类型：
--
作者：
Ale A;Crepin VF;Collins JW;Constantinou N;Habibzay M;Babtie AC;Frankel G;Stumpf MPH
在体内跟踪疾病进展对于开发针对细菌感染的治疗是必不可少的。光学成像已成为体内追踪细菌群体发育和治疗反应的核心工具。然而，为了准确理解从详细的死后观察得出的疾病机制方面的体内成像结果，需要两者之间的联系。在这里，我们开发了一个模型，提供了一个链接的调查柠檬酸杆菌感染，小鼠模型肠致病性大肠杆菌（EPEC）。我们连接体内疾病进展的C57 BL/6小鼠感染的生物发光细菌，成像使用光学断层扫描和X射线计算机断层扫描，死后测量结肠免疫细胞浸润。我们使用该模型来探索宿主免疫反应和细菌的变化，并评估对抗生素治疗的反应。开发的模型作为一种新的工具，用于识别和开发新的治疗干预措施。
Tracking disease progression in vivo is essential for the development of treatments against bacterial infection. Optical imaging has become a central tool for in vivo tracking of bacterial population development and therapeutic response. For a precise understanding of in vivo imaging results in terms of disease mechanisms derived from detailed postmortem observations, however, a link between the two is needed. Here, we develop a model that provides that link for the investigation of Citrobacter rodentium infection, a mouse model for enteropathogenic Escherichia coli (EPEC). We connect in vivo disease progression of C57BL/6 mice infected with bioluminescent bacteria, imaged using optical tomography and X-ray computed tomography, to postmortem measurements of colonic immune cell infiltration. We use the model to explore changes to both the host immune response and the bacteria and to evaluate the response to antibiotic treatment. The developed model serves as a novel tool for the identification and development of new therapeutic interventions.