A new agent of mycobacterial lymphadenitis in children: Mycobacterium heidelbergense sp. nov.
A new agent of mycobacterial lymphadenitis in children: Mycobacterium heidelbergense sp. nov.
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DOI：
10.1128/jcm.35.12.3203-3209.1997
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发表时间：
1997-12-01
影响因子：
9.4
通讯作者：
Bremer, HJ
中科院分区：
文献类型：
--
作者：
Haas, WH;Butler, WR;Bremer, HJ
非结核分枝杆菌性淋巴结炎在免疫功能正常的幼儿中日益引起临床问题。从1例复发性颈淋巴结炎患儿的淋巴组织中分离出2株生长缓慢、不发光的分枝杆菌（分离株2553/91和2554/91）。它可以区分从描述的分枝杆菌物种的生化，虽然它最接近的薄层色谱法和高效液相色谱法的分枝菌酸的马尔默分枝杆菌。该分离株的一个显著特征是其对体外抗结核药物（包括异烟肼）的高度敏感性。16 S rRNA基因序列的直接测定揭示了一个独特的序列，并将该菌株定位于与M. malmoense在一组缓慢生长的分枝杆菌中显示出与M. simiae的16 SrRNA基因序列同源性为99.6%。在16 SrRNA基因水平上与猿猴的DNA序列比较，DNA-DNA杂交（羟基磷灰石法）表明其DNA相关性小于40%，我们认为该生物是一个新种，并建议将其命名为M.海德堡。模式菌株2554/91的培养物作为菌株ATCC 51253保藏在美国典型培养物保藏中心。
Nontuberculous mycobacterial lymphadenitis presents an increasing clinical problem in immunocompetent young children, A slowly growing, nonphotochromogenic mycobacterium was recovered twice (isolates 2553/91 and 2554/91) from the lymphatic tissue of a child with recurrent cervical lymphadenitis. It could be differentiated biochemically from described Mycobacterium species, although it most closely resembled Mycobacterium malmoense by thin-layer chromatography and high-performance liquid chromatography of mycolic acids. A striking characteristic of the isolate was its high degree of susceptibility to antituberculous drugs in vitro, including isoniazid. Direct determination of the 16S rRNA gene sequence revealed a unique sequence and positioned the strain phylogenetically on a branch separate from M. malmoense within a group of slowly growing mycobacteria that show a high degree of similarity to M. simiae at the 16S rRNA gene level, Despite 99.6% sequence identity with M. simiae at the 16S rRNA gene level, DNA-DNA hybridization studies (hydroxyapatite method) demonstrated DNA relatedness of less than 40%, We conclude that this organism is a new species for which we propose the name M. heidelbergense. A culture of the type strain, strain 2554/91, has been deposited in the American Type Culture Collection as strain ATCC 51253.