Stiffness Tomography by Atomic Force Microscopy
Stiffness Tomography by Atomic Force Microscopy
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DOI：
10.1016/j.bpj.2009.05.010
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发表时间：
2009-07-22
影响因子：
3.4
通讯作者：
Kasas, Sandor
中科院分区：
文献类型：
--
作者：
Roduit, Charles;Sekatski, Serguei;Kasas, Sandor
原子力显微镜是一种方便的工具，在纳米尺度上探测活体样品。在其众多功能中，该仪器可以作为纳米压痕仪来收集有关样品机械性能的信息。在这种操作模式下，悬臂的变形显示为尖端进入样品的压痕深度的函数。用不同的理论模型拟合这条曲线，使我们能够估计压痕点处样品的杨氏模量。我们描述了据我们所知是一种新的技术来处理这些曲线，以区分埋在大部分样品中的不同刚度的结构。这种新的成像技术的工作原理已经通过有限元模型进行了验证，并成功地应用于活细胞。
The atomic force microscope is a convenient tool to probe living samples at the nanometric scale. Among its numerous capabilities, the instrument can be operated as a namo-indenter to gather information about the mechanical properties of the sample. In this operating mode, the deformation of the cantilever is displayed as a function of the indentation depth of the tip into the sample. Fitting this curve with different theoretical models permits us to estimate the Young's modulus of the sample at the indentation spot. We describe what to our knowledge is a new technique to process these curves to distinguish structures of different stiffness buried into the bulk of the sample. The working principle of this new imaging technique has been verified by finite element models and successfully applied to living cells.