Monitoring T-lymphocyte trafficking in tumors undergoing immune rejection
Monitoring T-lymphocyte trafficking in tumors undergoing immune rejection
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DOI：
10.1002/mrm.20717
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发表时间：
2005-12-01
影响因子：
3.3
通讯作者：
Brindle, KM
中科院分区：
文献类型：
--
作者：
Hu, DE;Kettunen, MI;Brindle, KM
从已经排斥表达鸡卵清蛋白的肿瘤（E.G7-OVA）的动物分离的活化的T细胞用柠檬酸化的超顺磁性氧化铁纳米颗粒标记，细胞内铁浓度高达0.5 pg/细胞。将这些标记的T细胞注射到经历免疫排斥的携带E.G7-OVA肿瘤的动物中导致这些细胞的肿瘤浸润，这在T-2加权MR图像中可检测为强度的不均匀降低。T细胞浸润证实了死后获得的肿瘤切片的免疫组化染色，并显示出共定位与铁，已被染色使用普鲁士蓝。肿瘤排斥与标记T细胞的摄取相关，因为标记T细胞的浸润仅在那些继续消退的肿瘤中观察到。这项技术应有助于阐明那些在介导肿瘤免疫排斥反应中很重要的因素。
Activated T cells, isolated from animals that had rejected a tumor (E.G7-OVA) expressing chicken ovalbumin, were labeled with citrated superparamagnetic iron oxide nanoparticles at an intracellular iron concentration of up to 0.5 pg/cell. Injection of these labeled T cells into animals bearing E.G7-OVA tumors undergoing immune rejection resulted in tumor infiltration of these cells, which was detectable as a heterogeneous decrease in intensity in T-2-weighted MR images. T-cell infiltration was confirmed by immunohistochemical staining of tumor sections obtained postmortem and was shown to colocalize with iron that had been stained using Prussian blue. Tumor rejection was correlated with the uptake of labeled T cells, since the infiltration of labeled T cells was only observed in those tumors that went on to regress. This technique should assist in the elucidation of those factors that are important in mediating tumor immune rejection.