Top down versus bottom up protein characterization by tandem high-resolution mass spectrometry
Top down versus bottom up protein characterization by tandem high-resolution mass spectrometry
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DOI：
10.1021/ja973655h
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发表时间：
1999-02-03
影响因子：
15
通讯作者：
McLafferty, FW
中科院分区：
文献类型：
--
作者：
Kelleher, NL;Lin, HY;McLafferty, FW
通过质谱（MS）对较大蛋白质进行表征尤其有前景，因为该信息可补充经典技术所获信息，并且仅需少至10⁻¹⁷摩尔的蛋白质即可获得。利用质谱来定位DNA衍生序列中的错误或修饰（翻译后修饰、衍生化活性位点等）通常涉及大量的蛋白酶解以产生……的肽段
Characterization of larger proteins by mass spectrometry (MS) is especially promising because the information complements that of classical techniques and can be obtained on as little as 10(-17) mol of protein. Using MS to localize errors in the DNA-derived sequence or modifications (posttranslational, derivatized active sites, etc.) usually involves extensive proteolysis to yield peptides of