Noninvasive study of radiation-induced oxidative damage using in vivo electron spin resonance
Noninvasive study of radiation-induced oxidative damage using in vivo electron spin resonance
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DOI：
10.1016/s0891-5849(00)00162-3
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发表时间：
2000-03-15
影响因子：
7.4
通讯作者：
Ozawa, T
中科院分区：
文献类型：
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作者：
Miura, Y;Ozawa, T
氮氧自由基注入全身表明在体内的电子自旋共振（ESR）的信号强度的消失。据报道，硝酰基的信号衰减速率受到各种类型的氧化应激的影响。我们用在体ESR研究了X射线照射对小鼠上腹部硝酰基信号衰减率的影响。在15戈伊照射后1 h，信号衰减率增加，预先给予辐射保护剂半胱胺可抑制这种增强。这些结果表明，整个小鼠的硝酰基信号衰减增强辐射诱导的氧化损伤。在体ESR系统探测硝酰基的信号衰减，可以提供一个非侵入性的技术，在活体辐射引起的氧化应激的研究。(C)2000 Elsevier Science Inc.
Nitroxyl radicals injected into a whole body indicate the disappearance of signal intensity of in vivo electron spin resonance (ESR). The signal decay rates of nitroxyl have reported to be influenced by various types of oxidative stress. We examined the effect of X-irradiation on the signal decay rate of nitroxyl in the upper abdomen of mice using in vivo ESR. The signal decay rates increased 1 h after 15 Gy irradiation, and the enhancement was suppressed by preadministration of cysteamine, a radioprotector. These results suggest that the signal decay of nitroxyl in whole mice is enhanced by radiation-induced oxidative damage. The in vivo ESR system probing the signal decay of nitroxyl could provide a noninvasive technique for the study of oxidative stress caused by radiation in a living body. (C) 2000 Elsevier Science Inc.