ABORTED GAMETOPHYTE 1 is required for gametogenesis in Arabidopsis
ABORTED GAMETOPHYTE 1 is required for gametogenesis in Arabidopsis
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ABORTED GAMETOPHYTE 1 是拟南芥配子发生所必需的。
DOI：
10.1111/jipb.12341
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发表时间：
2015-12-01
影响因子：
11.4
通讯作者：
Zhang, Xue-Qin
中科院分区：
文献类型：
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作者：
Cui, Hong-Hui;Liao, Hong-Ze;Zhang, Xue-Qin
在开花植物中，雌雄配子体的发生是有性生殖的关键步骤。尽管许多基因已被确定参与配子体发生过程，但配子体发生的遗传机制仍然知之甚少。本文报道了拟南芥配子体发生所必需的基因ABORTED GAMETOPHYTE 1 （AOG1）的特征。AOG1主要在生殖组织中表达，包括发育中的花粉粒和胚珠。AOG1蛋白与其他已记录的蛋白在氨基酸序列上没有明显的相似性，主要位于细胞核中。AOG1突变导致单核小孢子期花粉退化和胚囊严重缺陷，导致雄性和雌性生殖力显著降低。此外，分子分析表明，aog1突变体显著影响了配子体发生所需的几个基因的表达。我们的研究结果表明，AOG1在拟南芥花粉有丝分裂I （PMI）之前的配子体发生中起重要作用，可能是通过与其他基因的协同作用。
In flowering plants, the male and female gametogenesis is a crucial step of sexual reproduction. Although many genes have been identified as being involved in the gametogenesis process, the genetic mechanisms underlying gametogenesis remains poorly understood. We reported here characterization of the gene, ABORTED GAMETOPHYTE 1 (AOG1) that is newly identified as essential for gametogenesis in Arabidopsis thaliana. AOG1 is expressed predominantly in reproductive tissues including the developing pollen grains and ovules. The AOG1 protein shares no significant amino acid sequence similarity with other documented proteins and is located mainly in nuclei of the cells. Mutation in AOG1 caused degeneration of pollen at the uninucleate microspore stage and severe defect in embryo sacs, leading to a significant reduction in male and female fertility. Furthermore, the molecular analyses showed that the aog1 mutant significantly affected the expression of several genes, which are required for gametogenesis. Our results suggest that AOG1 plays important roles in gametogenesis at the stage prior to pollen mitosis I (PMI) in Arabidopsis, possibly through collaboration with other genes.