Angiogenic Potency of Amadori‐Glycated Phosphatidylethanolamine
Angiogenic Potency of Amadori‐Glycated Phosphatidylethanolamine
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DOI：
10.1196/annals.1333.048
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发表时间：
2005-06
影响因子：
5.2
通讯作者：
K. Nakagawa;Jeong‐Ho Oak;T. Miyazawa
中科院分区：
文献类型：
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作者：
K. Nakagawa;Jeong‐Ho Oak;T. Miyazawa
摘要：糖基化一直被认为参与糖尿病血管疾病。然而，没有关于脂质糖化对内皮功能障碍影响的报道。在这项研究中，我们评估了Amadori‐糖基化磷脂酰乙醇胺（Amadori‐PE），一种脂链糖基化化合物，是否影响培养的人脐静脉内皮细胞的增殖、迁移和管状形成。在低于5 μM的低浓度下，Amadori‐PE显著刺激了这三个参与血管生成的因子。此外，Amadori‐PE还刺激了基质金属蛋白酶- 2 （MMP‐2）的分泌，这是血管生成初始阶段的关键酶。我们的研究结果首次表明，Amadori‐PE可通过内皮细胞的血管生成能力引发血管疾病，从而在糖尿病微血管病变的发生和进展中发挥积极作用。
Abstract: Glycation has been thought to participate in diabetic vascular diseases. However, there are no reports about the effects of lipid glycation on endothelial dysfunction. In this study, we have evaluated whether Amadori‐glycated phosphatidylethanolamine (Amadori‐PE), a lipid‐linked glycation compound, affected proliferation, migration, and tube formation of cultured human umbilical vein endothelial cells. These three factors involved in angiogenesis were significantly stimulated by Amadori‐PE at a low concentration of less than 5 μM. Furthermore, Amadori‐PE also stimulated the secretion of matrix metalloproteinase‐2 (MMP‐2), a pivotal enzyme in the initial step of angiogenesis. Our results indicated for the first time that Amadori‐PE would elicit vascular disease through angiogenic potency on endothelial cells, thereby playing an active part in the development and progression of diabetic microangiopathy.