Transmissible and nontransmissible mutations induced by irradiating Arabidopsis thaliana pollen with γ-rays and carbon ions
Transmissible and nontransmissible mutations induced by irradiating Arabidopsis thaliana pollen with γ-rays and carbon ions
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DOI：
10.1534/genetics.104.033654
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发表时间：
2005-02-01
期刊：
影响因子：
3.3
通讯作者：
Nishimura, M
中科院分区：
文献类型：
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作者：
Naito, K;Kusaba, M;Nishimura, M
一项早期的遗传研究表明，大多数辐射引起的突变不会传给后代。在最近的植物分子研究中，主要是M-2植物或其后代，其中只含有可传递的突变，已被分析，但早期的结果意味着，这些研究是不够的，作为辐射诱导的突变的全面描述。为了在分子水平上研究低LET γ射线和高LET碳离子引起的辐射诱导突变，我们使用花粉照射方法和植物拟南芥来研究各种突变，包括非传递性突变。该分析显示，用γ射线（150-600戈伊）或碳离子（40-150戈伊）辐照诱导的大多数突变体携带高达> 6 Mbp的极大缺失，其中大多数未传递给后代。比较显示不同传递方式的突变体中的缺失区域，表明大缺失的非传递性可能是由于含有配子发育或存活所需的一个或多个基因的特定区域的缺失。
An early genetic study showed that most radiation-induced mutations are not transmitted to progeny. In recent molecular studies in plants, mainly M-2 plants or their progeny, which contain only transmissible mutations, have been analyzed, but the early results imply that these studies are insufficient as comprehensive descriptions of radiation-induced mutations. To study radiation-induced mutations caused by low-LET gamma-rays, and high-LET carbon ions at the molecular level, we used the pollen-irradiation method and the plant Arabidopsis thaliana to study various mutations, including non transmissible mutations. This analysis revealed that most mutants induced with irradiation with gamma-rays (150-600 Gy) or carbon ions (40-150 Gy) carried extremely large deletions of up to > 6 Mbp, the majority of which were not transmitted to progeny. Mutations containing 1- or 4-bp deletions, which were transmitted normally, were also found. Comparison of the deleted regions in the mutants showing various manners of transmission suggests that the non-transmissibility of the large deletions may be due to the deletion of a particular region that contains a gene or genes required for gamete development or viability.