Transcriptome profiling and digital gene expression by deep-sequencing in normal/regenerative tissues of planarian Dugesia japonica
Transcriptome profiling and digital gene expression by deep-sequencing in normal/regenerative tissues of planarian Dugesia japonica
复制标题
通过深度测序对涡虫正常/再生组织进行转录组分析和数字基因表达
DOI：
10.1016/j.ygeno.2011.02.002
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发表时间：
2011-06-01
期刊：
影响因子：
4.4
通讯作者：
Zhang, Shou-Tao
中科院分区：
文献类型：
--
作者：
Qin, Yun-Fei;Fang, Hui-Min;Zhang, Shou-Tao
涡虫表现出再生失去的身体部位的非凡能力，这归因于大量被称为新生细胞的多能体干细胞。在本文中，我们报告了通过高通量测序获得的涡虫亚种 Dugesia japonica 的转录组序列。此外，我们通过使用基于标签的数字基因表达（DGE）系统研究了不同再生时期的转录组变化。结果，获得了 11,913,548 个转录组测序读数。最后，这些读段最终被组装成 37,218 个独特的 unigene。这些组装的unigenes用各种方法进行了注释。使用基于标签的 DGE 系统研究了涡虫再生期间的转录组变化。我们对每个样本获得了超过350万个标签的测序深度，并鉴定出了再生各个阶段的大量差异表达基因。这些结果为涡虫发育的研究，特别是其再生进展的研究提供了相当全面的分子生物学背景。 (C) 2011 Elsevier Inc. 保留所有权利。
Planarians exhibit an extraordinary ability to regenerate lost body parts which is attributed to an abundance of pluripotent somatic stem cells called neoblasts. In this article, we report a transcriptome sequence of a Planaria subspecies Dugesia japonica derived by high-throughput sequencing. In addition, we researched transcriptome changes during different periods of regeneration by using a tag-based digital gene expression (DGE) system. Consequently, 11,913,548 transcriptome sequencing reads were obtained. Finally, these reads were eventually assembled into 37,218 unique unigenes. These assembled unigenes were annotated with various methods. Transcriptome changes during planarian regeneration were investigated by using a tag-based DGE system. We obtained a sequencing depth of more than 3.5 million tags per sample and identified a large number of differentially expressed genes at various stages of regeneration. The results provide a fairly comprehensive molecular biology background to the research on planarian development, particularly with regard to its regeneration progress. (C) 2011 Elsevier Inc. All rights reserved.