Platelet protein phosphorylation, elevation of cytosolic calcium, and inositol phospholipid breakdown in platelet activation induced by plasmin.
Platelet protein phosphorylation, elevation of cytosolic calcium, and inositol phospholipid breakdown in platelet activation induced by plasmin.
复制标题
纤溶酶诱导的血小板活化中的血小板蛋白磷酸化、胞浆钙升高和肌醇磷脂分解。
DOI：
10.1172/jci112576
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
E W Salzman
中科院分区：
文献类型：
--
作者：
A I Schafer;A. K. Maas;J A Ware;P C Johnson;S. Rittenhouse;E W Salzman
Studies have been performed on the biochemical mechanism of platelet activation induced by the fibrinolytic protease plasmin. In washed human platelets, greater than or equal to 1.0 caseinolytic units (CU/ml plasmin induced aggregation. Platelet [14C]serotonin release was stimulated by 1.0 CU/ml plasmin to an extent comparable to that induced by 1.0 U/ml thrombin. A dose- and time-dependent phosphorylation of the platelet 47,000- and 20,000-kD proteins was noted in 32PO4-labeled platelets incubated with plasmin; phosphorylation was not affected by extracellular Ca2+, but was completely inhibited by an increase in platelet cyclic AMP. Phosphorylation of these platelet proteins suggested that plasmin may act on platelets by stimulating a rise in cytosolic calcium concentration ([Cai2+]) and activating inositol phospholipid-dependent phospholipase C and protein kinase C. Using both quin2 fluorescence and aequorin luminescence as indicators, plasmin was found to elevate platelet [Cai2+] in the presence or absence of extracellular Ca2+. Phospholipase C activation was shown by the generation of [3H]diglyceride in [3H]arachidonic acid-labeled platelets and [32P]phosphatidic acid in 32PO4 labeled platelets exposed to plasmin. Plasmin did not induce formation of thromboxane A2 (TXA2). Only small amounts of this eicosanoid were detected late in the time course after plasmin stimulation. Our results indicate that plasmin causes platelet aggregation and secretion associated with phosphorylation of the 47,000- and 20,000-kD proteins, Ca2+ mobilization, and phospholipase C and protein kinase C activation.
血小板表面纤溶酶原的结合和激活。
DOI：
--
发表时间：
1985
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Miles,LA;Plow,EF
通讯作者：
Plow,EF
钙和磷酸肌醇在某些激素和神经递质的作用中的作用。
DOI：
10.1172/jci111886
发表时间：
1985
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Exton,JH
通讯作者：
Exton,JH
纤溶酶对血小板糖蛋白 Ib-von Willebrand 因子相互作用的影响。
影响因子：
20.3
作者：
Adelman,B;Michelson,AD;Loscalzo,J;Greenberg,J;Handin,RI
通讯作者：
Handin,RI