Amperometric choline biosensor based on multiwalled carbon nanotubes/zirconium oxide nanoparticles electrodeposited on glassy carbon electrode.
Amperometric choline biosensor based on multiwalled carbon nanotubes/zirconium oxide nanoparticles electrodeposited on glassy carbon electrode.
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DOI：
10.1016/j.ab.2012.04.027
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发表时间：
2012-08
影响因子：
2.9
通讯作者：
S. Pundir;N. Chauhan;J. Narang;C. Pundir
中科院分区：
文献类型：
--
作者：
S. Pundir;N. Chauhan;J. Narang;C. Pundir
A bienzymatic choline biosensor was constructed by coimmobilizing acetylcholinesterase (AChE) and choline oxidase (ChO) onto nanocomposite of carboxylated multiwalled carbon nanotubes (c-MWCNTs) and zirconium oxide nanoparticles (ZrO2NPs) electrodeposited on the surface of a glassy carbon electrode (GCE) and using it (AChE–ChO/c-MWCNT/ZrO2NPs/GCE) as working electrode, Ag/AgCl as reference electrode, and Pt wire as auxiliary electrode connected through a potentiostat. The enzyme electrode was characterized by scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy, and cyclic voltammetry (CV) studies, optimized, and evaluated. The biosensor exhibited optimum response within 4s at +0.2V, pH 7.4, and 25°C. The detection limit and working range of the biosensor were 0.01μM and 0.05 to 200μM, respectively. The half-life of the enzyme electrode was 60days at 4°C. The serum choline level, as measured by the biosensor, was 9.0 to 12.8μmol/L (with a mean of 10.81μmol/L) in apparently healthy persons and 5.0 to 8.4μmol/L (with a mean of 6.53μmol/L) in persons suffering from Alzheimer’s disease. The enzyme electrode was unaffected by a number of serum substances.
