Evidence for p62 aggregate formation: Role in cell survival
Evidence for p62 aggregate formation: Role in cell survival
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DOI：
10.1016/j.febslet.2005.08.010
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发表时间：
2005-09-12
期刊：
影响因子：
3.5
通讯作者：
Wooten, MW
中科院分区：
文献类型：
--
作者：
Paine, MG;Babu, JR;Wooten, MW
多聚泛素结合蛋白p62已被证明定位于几种类型疾病常见的聚集体中。在这里，我们报告说，p62形成独立的纤维聚集体在体外的时间和浓度依赖性的方式。p62的FTIR光谱和ThT荧光测定揭示了与天然蛋白质相比，随着聚集体的形成，β-折叠含量增加。通过透射电子显微镜观察聚集体的纤维性质。p62在HEK细胞中的过表达导致聚集体形成，其可以保护细胞免于凋亡。总之，这些结果表明，p62纤维可能会影响细胞活力，并表明p62在侵略形成的重要作用。(c)2005年欧洲生物化学学会联合会。Elsevier B. V.出版，保留所有权利。
The polyubiquitin-binding protein p62 has been shown to localize in aggregates common to several types of diseases. Here, we report that p62 forms independent fibrillar aggregates in vitro in a time- and concentration-dependent manner. FTIR spectra and ThT fluorescence assay of p62 reveals increased beta-sheet content as aggregates form compared to the native protein. The fibrillar nature of the aggregates was observed by transmission electron microscopy. Overexpression of p62 in HEK cells results in aggregate formation that may protect cells from apoptosis. Altogether, these results suggest that p62 fibrils may influence cell viability and indicates an important role for p62 in aggresome formation. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.