Fast and Efficient Measurement of Clinical and Biological Samples Using Immunoassay-Based Multiplexing Systems.
Fast and Efficient Measurement of Clinical and Biological Samples Using Immunoassay-Based Multiplexing Systems.
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DOI：
10.1007/978-1-0716-0223-2_6
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发表时间：
2020-01-01
期刊：
影响因子：
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通讯作者：
Di, Y Peter
中科院分区：
文献类型：
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作者：
Zhang, Yingze;Li, Xiaoyun;Di, Y Peter
免疫测定是检测测试样品中抗体或抗原表达的最常用的生物医学技术之一。酶联免疫吸附测定（ELISA）已用于多种应用，包括诊断工具和质量控制。然而，ELISA的主要限制之一是其缺乏多重能力，因此当需要测量具有有限数量的样品（例如来自新生儿或婴儿的血液或生物样品）的多个决定因素时，ELISA可能不是有效的诊断工具。尽管在测定测量中类似于ELISA，但多重平台如基于珠的Luminex和基于多阵列的MSD（Meso Scale Discovery）被广泛用于从单个分析测量多个生物标志物。Luminex是一种基于xMAP的技术，它结合了几种不同的技术，可以从单个样品中高效准确地测量多种分析物。可以实现多路复用，因为多达100个不同的Luminex颜色编码的微球珠组可以用特定生物测定的特异性试剂包被，从而允许从样品中捕获和检测特定分析物。使用多阵列和电化学发光技术，MSD平台提供了与ELISA中观察到的一致性相似的多重能力。Luminex和MSD系统均可分析的各种生物样本包括血清、血浆、组织和细胞裂解物、唾液、痰液和支气管肺泡灌洗（BAL）。最常见的基于Luminex和MSD的测定是检测一组组合的细胞因子，以提供用于诊断目的的细胞因子表达谱的测量。
Immunoassay is one of the most commonly used biomedical techniques to detect the expression of an antibody or an antigen in a test sample. Enzyme-linked immunosorbent assay (ELISA) has been used for a variety of applications including diagnostic tools and quality controls. However, one of the main limitations of ELISA is its lack of multiplexing ability, so ELISA may not be an efficient diagnostic tool when a measurement of multiple determinants is needed for samples with limited quantity such as blood or biological samples from newborns or babies. Although similar to ELISA in assay measurement, multiplex platforms such as bead-based Luminex and multi-array-based MSD (Meso Scale Discovery) are widely used to measure multiple biomarkers from a single analysis. Luminex is a xMAP-based technology that combines several different technologies to provide an efficient and accurate measurement of multiple analytes from a single sample. The multiplexing can be achieved because up to 100 distinct Luminex color-coded microsphere bead sets can be coated with a reagent specific to a particular bioassay, allowing the capture and detection of specific analytes from a sample. Using Multi-array and electrochemiluminescence technologies, the MSD platform provides the multiplex capability with similar consistence as observed in ELISA. Various biological samples that can be analyzed by both Luminex and MSD systems include serum, plasma, tissue and cell lysate, saliva, sputum, and bronchoalveolar Lavage (BAL). The most common Luminex and MSD-based assays are to detect a combined set of cytokines to provide a measurement of cytokine expression profiling for a diagnostic purpose.