Isolation and characterization of nine polymorphic microsatellite loci in the endangered shrub Disanthus cercidifolius var. longipes (Hamamelidaceae)

Isolation and characterization of nine polymorphic microsatellite loci in the endangered shrub Disanthus cercidifolius var. longipes (Hamamelidaceae)
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DOI:
10.1111/j.1755-0998.2009.02564.x
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发表时间:
2009-05-01
影响因子:
7.7
通讯作者:
Huang, Hongwen
Huang, Hongwen
中科院分区:
生物学1区
文献类型:
--
作者:
Gao, Puxin;Yang, Aihong;Huang, Hongwen

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从大叶石竹AC富集库中分离到9个微卫星座位,并对其进行了分析。拉吉普斯。对来自一个自然群体的24个个体进行了微卫星多态性研究。观察到的每个座位的等位基因数量在2到4个之间。观察杂合度为0.17~0.92,预期杂合度为0.16~0.72。这些多态微卫星座位为正在进行的大叶杜鹃种群遗传学研究提供了有用的工具。拉吉普斯。
Nine polymorphic microsatellite loci were isolated and characterized from an AC-enriched genomic library of Disanthus cercidifolius var. longipes. Microsatellite polymorphism was investigated using 24 individuals from one natural population. The observed number of alleles per locus ranged from two to four. Observed and expected heterozygosities ranged from 0.17 to 0.92 and from 0.16 to 0.72, respectively. These polymorphic microsatellite loci provide useful tools for the ongoing population genetic studies of D. cercidifolius var. longipes.