Transcriptional Association between mRNAs and Their Paired Natural Antisense Transcripts Following Fusarium oxysporum Inoculation in Brassica rapa L.
Transcriptional Association between mRNAs and Their Paired Natural Antisense Transcripts Following Fusarium oxysporum Inoculation in Brassica rapa L.
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DOI：
10.3390/horticulturae8010017
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发表时间：
2022-01-01
期刊：
影响因子：
3.1
通讯作者：
Osabe, Kenji
中科院分区：
文献类型：
--
作者：
Akter, Mst. Arjina;Mehraj, Hasan;Osabe, Kenji
长非编码RNAs(Long Non Coding RNAs，LncRNAs)在非生物和生物胁迫反应中发挥着重要作用，但对植物中LncRNA表达调控机制的研究还很有限。本研究探讨了白菜组蛋白H3K4me3和H3K36me3两种活性修饰与lncRNA表达水平的关系。这两个组蛋白标记都在编码lncRNAs的染色质区域富含，尤其是在转录起始点附近。长基因间非编码RNA的转录水平与H3K4me3和H3K36me3的转录水平呈正相关，而在自然反义RNA和内含子非编码RNA中未观察到这种关联。由于在生物胁迫处理下mRNAs和成对NAT的协同表达已被鉴定，因此研究了尖孢镰刀菌根腐病后mRNAs和其成对NAT之间的转录关系。对粘连杆菌(Foc)接种进行检测。在FOC接种后，观察到mRNAs与其配对NAT之间的表达水平呈正相关。这种关联适用于几个与防御反应相关的基因和它们的NAT对。这些结果表明，mRNAs和成对NAT的协同表达在对Foc的防御反应中发挥了作用。
Long noncoding RNAs (lncRNAs) play important roles in abiotic and biotic stress responses; however, studies on the mechanism of regulation of lncRNA expression are limited in plants. The present study examined the relationship between lncRNA expression level and two active histone modifications (H3K4me3 and H3K36me3) in Brassica rapa. Both histone marks were enriched in the chromatin regions encoding lncRNAs, especially around the transcription start site. The transcription level of long intergenic noncoding RNAs was positively associated with the level of H3K4me3 and H3K36me3, while this association was not observed in natural antisense RNAs (NATs) and intronic noncoding RNAs. As coordinate expression of mRNAs and paired NATs under biotic stress treatment has been identified, the transcriptional relationship between mRNAs and their paired NATs following Fusarium oxysporum f. sp. conglutinans (Foc) inoculation was examined. A positive association of expression levels between mRNAs and their paired NATs following Foc inoculation was observed. This association held for several defense-response-related genes and their NAT pairs. These results suggest that coordinate expression of mRNAs and paired NATs plays a role in the defense response against Foc.