Decellularization Strategies for Regenerating Cardiac and Skeletal Muscle Tissues.
Decellularization Strategies for Regenerating Cardiac and Skeletal Muscle Tissues.
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DOI：
10.3389/fbioe.2022.831300
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发表时间：
2022
影响因子：
5.7
通讯作者：
Nakayama KH
中科院分区：
文献类型：
--
作者：
Tan YH;Helms HR;Nakayama KH
心血管疾病是全球死亡的主要原因，每年约有1790万人死亡。肌肉骨骼疾病影响全球超过17.1亿人，是导致残疾的主要原因。这两个领域代表了巨大的全球健康负担，由于缺乏功能恢复性治疗方案而长期存在。再生医学和组织工程领域为开发修复受损或患病组织的疗法提供了巨大的希望。脱细胞组织和细胞外基质是再生生物材料的基石，已经在临床上使用了几十年，许多已经获得FDA的批准。在这篇综述中，我们首先讨论和比较用于从心肌和骨骼肌中制备脱细胞组织和ECM的方法。我们重点关注不同的生物物理特性，如空间形貌，细胞外基质成分和机械特性如何影响再生医学背景下的细胞行为和功能。最后，我们描述了新兴的研究，并预测了脱细胞心肌和骨骼肌的未来高影响力应用，这将推动新的和有效的再生疗法。
Cardiovascular disease is the leading cause of death worldwide and is associated with approximately 17.9 million deaths each year. Musculoskeletal conditions affect more than 1.71 billion people globally and are the leading cause of disability. These two areas represent a massive global health burden that is perpetuated by a lack of functionally restorative treatment options. The fields of regenerative medicine and tissue engineering offer great promise for the development of therapies to repair damaged or diseased tissues. Decellularized tissues and extracellular matrices are cornerstones of regenerative biomaterials and have been used clinically for decades and many have received FDA approval. In this review, we first discuss and compare methods used to produce decellularized tissues and ECMs from cardiac and skeletal muscle. We take a focused look at how different biophysical properties such as spatial topography, extracellular matrix composition, and mechanical characteristics influence cell behavior and function in the context of regenerative medicine. Lastly, we describe emerging research and forecast the future high impact applications of decellularized cardiac and skeletal muscle that will drive novel and effective regenerative therapies.