Pharmacokinetics of oxolinic acid in black tiger shrimp, Penaeus monodon Fabricius, and the effect of cooking on residues
Pharmacokinetics of oxolinic acid in black tiger shrimp, Penaeus monodon Fabricius, and the effect of cooking on residues
复制标题
DOI：
10.1111/j.1365-2109.2006.01500.x
复制
发表时间：
2006-06
影响因子：
2
通讯作者：
K. Uno;T. Aoki;Walai Kleechaya;L. Ruangpan;V. Tanasomwang
中科院分区：
文献类型：
--
作者：
K. Uno;T. Aoki;Walai Kleechaya;L. Ruangpan;V. Tanasomwang
本文研究了草酸(OA)在斑节对虾(Penaeus Monodon Fabricius)体内的药代动力学和生物利用度。在28℃、291C、盐水(盐度10gL 1)中，经鼻窦给药(10mgkg~1)和经口给药(50mgkg~(-1))后，研究了虾体内残留奥沙林酸(OA)的净变化。OAafterintra-sinusdosingwerebestdescribedbyatwo-com-partment开放式血淋巴浓度分布和消除半衰期分别为0.84和17.7h。稳态分布表观体积为2061mL·kg~(-1)，总清除量为90.1mL·kg~(-1)·h，口服后的生物利用度为7.9%。血淋巴峰值浓度、血淋巴浓度达峰时间和消除半衰期分别为4.20 mg·L-1、4h和19.8h。氧亚麻酸在给药后4h达到最大值(肌肉1.76mgg 1，贝壳8.17mgg)，然后下降，消除半衰期分别为20.2和21.9h。关键词：药代动力学，草酸，斑节对虾，黑虎虾，生物利用度，烹饪简介在东南亚，集约化养虾在过去的十年里发展迅速。泰国黑虎对虾是泰国重要的经济出口国，大部分产品消耗在美国和日本。近年来，由弧菌引起的疾病对泰国养殖业造成了严重的经济损失。为了减少疾病问题，广泛使用了化疗药物。在对虾养殖中使用化疗药物会对水环境造成影响，并有可能导致健康风险，因为使用抗菌剂会导致theshrimpsoldforconsumption.Oneofthemajorenvir-onmental中的药物残留导致细菌耐药性的产生。因此，theproperuseofdrugsisstronglyrecommendedtoavoidsuch出现了问题。了解药物动力学和残留消耗对于最大限度地减少与药物残留有关的人类风险和药物对环境的影响是重要的。目前，恶唑酸(OA)是泰国对虾养殖场最常用的制剂。Phar-macokineticpro¢leofOAhaspreviouslybeenstudiedin彩虹鲑鱼Oncorhynchus mykis(Walbaum)(Bj˛rklundBHustvedtSKlei-Now，Jarboe，Shoemaker&Greenless 1994)，大西洋鲑鱼Salmo salar L.(Hustvedt，Salte&Vassvik 1991；Rogstad，EllingsenSMartinsen&Horsberg1995；Samuelsen，Ervik，Pursell&Smith2000)，Channel elcat，Ictaluruspot talusL.(Kleinow
AbstractThis study examined the pharmacokinetics andbioavailability of oxolinic acid (OA) in black tigershrimpPenaeus monodon Fabricius,inbrackishwater(salinity 10gL 1 )at28^291C, after intra-sinus (10mgkg 1 ) and oral (50mgkg ) administration andalso investigated the net changes of OA residuesin the shrimp after cooking (boiling, baking andfrying).The haemolymph concentrations of OAafterintra-sinusdosingwerebestdescribedbyatwo-com-partment open model.The distribution and elimina-tion half-lives were 0.84 and17.7h respectively. Theapparent volume of distributionata steadystate andthe total body clearance were estimated to be2061mL kg 1 and 90.1mLkg h respectively.Thebioavailabilityof OAafteranoraladministrationwas 7.9%. The peak haemolymph concentration,the time to peak haemolymph concentration andthe elimination half-life after oral administrationwere 4.20mgmL 1 ,4h and19.8h respectively. Oxo-linic acid muscle and shell levels increased to amaximum (muscle1.76 mgg 1 and shell 8.17mgg )at 4h post administration and then decreased withthe elimination half-life value of 20.2 and 21.9hrespectively. Residual OA in muscle and shell wasreduced by 20^30% by each cooking procedureexamined.Keywords: pharmacokinetics, oxolinicacid, Pena-eus monodon, black tiger shrimp, bioavailability,cookingIntroductionIn Southeast Asia, intensive shrimp farming has de-veloped rapidly inthelast decade. Blacktiger shrimpPenaeusmonodon Fabriciusisaneconomicallyimpor-tant export inThailand, and the greater part of pro-ductionis consumedbytheUSAandJapan.Recently,diseases caused byVibrio bacteria are a serious pro-blem causing economic damage to theThai shrimpindustry.Toreduce diseaseproblems, awide range ofchemotherapeutics has been used. The use of che-motherapeuticdrugs in shrimpfarmingcan haveanimpact on the aquatic environment and potentiallycause health risks because of drug residues in theshrimpsoldforconsumption.Oneofthemajorenvir-onmental eiects by using antibacterial agents isdevelopment of bacterial resistance. Therefore, theproperuseofdrugsisstronglyrecommendedtoavoidsuch problems. Knowledge of pharmacokinetics andresidue depletion is important to minimize humanrisk associated with drug residues and the environ-mental impactof thedrugs.Currently,oxolinicacid(OA)isthemostcommonlyused agent in Thai shrimp culture farms. The phar-macokineticpro¢leofOAhaspreviouslybeenstudiedin rainbow trout Oncorhynchus mykiss (Walbaum)(Bj˛rklundBHustvedtSKlei-now, Jarboe, Shoemaker & Greenless 1994), Atlanticsalmon Salmo salar L. (Hustvedt, Salte & Vassvik1991;Rogstad,EllingsenSMartinsen& Horsberg1995; Samuelsen, Ervik, Pursell & Smith2000),channelcat¢shIctaluruspunctatusL.(Kleinow