Genomic Approaches to Zebrafish Cancer.
Genomic Approaches to Zebrafish Cancer.
复制标题
斑马鱼癌症的基因组学方法。
DOI：
10.1007/978-3-319-30654-4_6
复制
发表时间：
2016
影响因子：
--
通讯作者：
White,RichardM
中科院分区：
文献类型：
--
作者：
White,RichardM
斑马鱼已经成为研究癌症生物学的重要模型。DNA、RNA和染色质异常的鉴定可以深入了解肿瘤发生的机制，并且有许多技术可以分析这些肿瘤的基因组。在这里，我提出了一个可用的技术分析斑马鱼肿瘤基因组，包括基于阵列的方法以及下一代测序技术的概述。我还讨论了斑马鱼肿瘤基因组可以使用跨物种癌基因组学与人类基因组进行比较的方式，该基因组学可以过滤基因组噪音并最终发现恶性肿瘤的中心驱动因素。最后，我讨论了下游分析工具，包括网络分析，可以帮助组织成连贯的生物框架，然后可以进一步调查的变化。
The zebrafish has emerged as an important model for studying cancer biology. Identification of DNA, RNA and chromatin abnormalities can give profound insight into the mechanisms of tumorigenesis and the there are many techniques for analyzing the genomes of these tumors. Here, I present an overview of the available technologies for analyzing tumor genomes in the zebrafish, including array based methods as well as next-generation sequencing technologies. I also discuss the ways in which zebrafish tumor genomes can be compared to human genomes using cross-species oncogenomics, which act to filter genomic noise and ultimately uncover central drivers of malignancy. Finally, I discuss downstream analytic tools, including network analysis, that can help to organize the alterations into coherent biological frameworks that can then be investigated further.