Effect of in vitro treatment with indomethacin on mouse granulocyte-macrophage colony-forming cells in culture (CFUC). Possible role of prostaglandins.
Effect of in vitro treatment with indomethacin on mouse granulocyte-macrophage colony-forming cells in culture (CFUC). Possible role of prostaglandins.
复制标题
吲哚美辛体外处理对培养的小鼠粒细胞-巨噬细胞集落形成细胞（CFUC）的影响。
作者：
J. Fontagné;M. Adolphe;M. Sémichon;L. Zizine;P. Lechat
本文观察了消炎痛(每天6 mg/kg，连续给药2、3、4天)对小鼠骨髓细胞分化的影响，观察了GM-CSF对CFUC增殖的影响，以及胸腺嘧啶核苷自杀后CFUC的增殖状态。治疗4d后，可识别的骨髓前体细胞数量增加，集落形成能力和S期CFUC数量均增加。这些数据表明，吲哚美辛引起了承诺干细胞室的增殖。骨髓细胞经消炎痛处理后前列腺素E_2含量的减少可以解释观察到的增生现象。体内实验结果与体外实验结果一致，表明PG对髓系祖细胞的增殖和分化具有一定的调控作用。
The effect of indomethacin (6 mg/kg daily orally for 2, 3 or 4 days) on myelopoiesis was studied in mice by estimating 1) differential cell counts of bone marrow, 2) proliferation of the CFUC in presence of GM-CSF, 3) proliferative state of CFUC after tritiated thymidine suicide. After 4 days of treatment, a rise in the recognizable myeloid precursors, an increase of both the colony forming capacity and the number of CFUC in S-phase were observed. These data suggest that indomethacin produced an hyperplasia of the committed stem cell compartment. The decrease of PGE2 amounts in bone marrow cells following treatment with indomethacin could explain the hyperplasia observed. These in vivo results are in accordance with the in vitro data which show that PG could control the proliferation and differentiation of myeloid progenitor cells.