Low diversity of Alkhurma hemorrhagic fever virus, Saudi Arabia, 1994-1999
Low diversity of Alkhurma hemorrhagic fever virus, Saudi Arabia, 1994-1999
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DOI：
10.3201/eid1105.041298
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发表时间：
2005-05-01
影响因子：
11.8
通讯作者：
de Lamballerie, X
中科院分区：
文献类型：
--
作者：
Charrel, RN;Zaki, AM;de Lamballerie, X
阿尔库玛出血热病毒（黄病毒属，AHFV）最近被确定为沙特阿拉伯病毒性出血热的病原体，并在血清学和遗传学上被定性为Kyasanur森林病病毒（KFDV）的一种变异基因型。由于病毒诊断和疫苗开发可能受到遗传多样性的阻碍，本研究旨在解决AHFV遗传异质性。对1994 ~ 1999年从沙特阿拉伯住院患者分离的11株菌株进行包膜、NS3和NS5基因测序。同源序列进行比较，并用于寻找反映与空间性、时间性、感染途径和疾病预后相关的特定进化模式。遗传分析显示多样性低，表明微进化缓慢。分化时间评估表明，AHFV和KFDV祖先谱系在66-177年前发生分化，所研究的AHFV株内观察到的多样性反映了4- 72年的进化周期。
Alkhurma hemorrhagic fever virus (genus Flavivirus, AHFV) was recently identified as the agent of a viral hemorrhagic fever in Saudi Arabia and characterized serologically and genetically as a variant genotype of Kyasanur Forest disease virus (KFDV). Since viral diagnosis and vaccine development may be hindered by genetic diversity, this study was intended to address AHFV genetic heterogeneity. Eleven strains isolated from hospitalized patients from 1994 to 1999 in Saudi Arabia were sequenced in the envelope, NS3, and NS5 genes. Homologous sequences were compared and used to look for patterns reflecting specific evolution associated with spatiality, temporality, infection pathway, and disease prognosis. Genetic analyses showed low diversity, which suggests a slow microevolution. Evaluation of divergence times showed that AHFV and KFDV ancestral lineage diverged 66-177 years ago, and the diversity observed within the studied AHFV strains reflected a 4- to 72-year period of evolution.