New replicable anxiety-related measures of wall vs. center behavior of mice in the open field
New replicable anxiety-related measures of wall vs. center behavior of mice in the open field
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DOI：
10.1152/japplphysiol.00148.2004
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发表时间：
2004-07-01
影响因子：
3.3
通讯作者：
Golani, I
中科院分区：
文献类型：
--
作者：
Lipkind, D;Sakov, A;Golani, I
焦虑是一种被广泛研究的精神疾病，被认为是一种复杂的多维现象。焦虑动物模型的敏感行为识别对于阐明焦虑的行为成分和介导它们的生理过程至关重要。常用的焦虑行为范式通常只包括几个自动收集的措施;这些措施并没有穷尽动物表现出的行为丰富性，因此可能遗漏了准备工作之间的重要差异。本研究的目的是扩大剧目的自动收集的措施，在经典的测试焦虑：行为在开放领域的墙壁。我们提出了一种算法，基于软件的探索的探索策略，它自动分区的鼠标路径到本质上定义的移动模式附近的墙壁和中心。这些模式用于设计新的端点，这些端点提供了对墙附近和中心的行为的各个方面的清晰描述。根据三个实验室中C57 BL/6 J和DBA/2 J小鼠的试验数据，设计了16个新的终点。在另一个数据集上评价了所有终点的应变差异，以评估其有效性，并发现其保持稳定。发现16个终点中的10个以可复制的方式区分两种菌株。整个终点集可以用于各种遗传和药理学模型的焦虑与良好的前景，提供精细的歧视，在一个可复制的方式。
Anxiety is a widely studied psychiatric disorder and is thought to be a complex and multidimensional phenomenon. Sensitive behavioral discrimination of animal models of anxiety is crucial for the elucidation of the behavioral components of anxiety and the physiological processes that mediate them. Commonly used behavior paradigms of anxiety usually include only a few automatically collected measures; these do not exhaust the behavioral richness exhibited by animals, thus perhaps missing important differences between preparations. The aim of the present study was to expand the repertoire of automatically collected measures in a classical test of anxiety: behavior in relation to the wall in the open field. We present an algorithm, based on the Software for the Exploration of Exploration strategy, which automatically partitions the mouse path into intrinsically defined patterns of movement near the wall and in the center. These patterns are used to design new end points, which provide an articulated description of various aspects of behavior near the wall and in the center. Sixteen new end points were designed with data from C57BL/6J and DBA/2J mice tested in three laboratories. The strain differences in all end points were evaluated on another data set to assess their validity and were found to remain stable. Ten of the sixteen end points were found to discriminate between the two strains in a replicable manner. The entire set of end points can be used on various genetic and pharmacological models of anxiety with good prospects of providing fine discrimination in a replicable manner.