2 FORMS OF MOUSE SYNTROPHIN, A 58-KD DYSTROPHIN-ASSOCIATED PROTEIN, DIFFER IN PRIMARY STRUCTURE AND TISSUE DISTRIBUTION
2 FORMS OF MOUSE SYNTROPHIN, A 58-KD DYSTROPHIN-ASSOCIATED PROTEIN, DIFFER IN PRIMARY STRUCTURE AND TISSUE DISTRIBUTION
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DOI：
10.1016/0896-6273(93)90157-m
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发表时间：
1993-09-01
期刊：
影响因子：
16.2
通讯作者：
FROEHNER, SC
中科院分区：
文献类型：
--
作者：
ADAMS, ME;BUTLER, MH;FROEHNER, SC
Syntrophin是一种分子量为58 kD的外源性膜蛋白，主要集中在神经肌肉接头的突触后部位，可能参与乙酰胆碱受体的聚集。在肌肉和非肌肉组织中，syntrophin与肌营养不良蛋白、肌营养不良蛋白和肌营养不良蛋白羧基末端区域的两个同源物相关。我们已经分离出三个编码电鳐和小鼠突触营养因子的cDNA。Torpedo cDNA编码全长蛋白，并且在北方印迹上识别3.5 kb mRNA。这两种小鼠突触营养蛋白cDNA是不同基因的产物，但编码的蛋白质具有50%的同一性。Syntrophin-1 mRNA（2.2 kb）在骨骼肌中表达水平最高。Syntrophin-2 mRNA（2.2、5.0和10 kb）在检查的所有小鼠组织中表达。这些表达模式表明，syntrophin-1和syntrophin-2可能与肌营养不良蛋白家族的不同成员。
Syntrophin, a 58 kd extrinsic membrane protein, is concentrated at postsynaptic sites at the neuromuscular junction and may be involved in clustering acetylcholine receptors. In muscle and nonmuscle tissues, syntrophin is associated with dystrophin, utrophin, and two homologs of the dystrophin carboxy-terminal region. We have isolated three cDNAs encoding Torpedo and mouse syntrophins. The Torpedo cDNA encodes a full-length protein, and on Northern blots recognizes a 3.5 kb mRNA. The two mouse syntrophin cDNAs are products of separate genes but encode proteins that share 50% identity. Syntrophin-1 mRNA (2.2 kb) is expressed at highest levels in skeletal muscle. Syntrophin-2 mRNAs (2.2, 5.0, and 10 kb) are expressed in all mouse tissues examined. These patterns of expression suggest that syntrophin-1 and syntrophin-2 may associate with different members of the dystrophin family.