The transforming growth factor-β superfamily cytokine macrophage inhibitory cytokine-1 is present in high concentrations in the serum of pregnant women
The transforming growth factor-β superfamily cytokine macrophage inhibitory cytokine-1 is present in high concentrations in the serum of pregnant women
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DOI：
10.1210/jc.85.12.4781
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发表时间：
2000-12-01
影响因子：
5.8
通讯作者：
Breit, SN
中科院分区：
文献类型：
--
作者：
Moore, AG;Brown, DA;Breit, SN
巨噬细胞抑制性细胞因子-1（macrophage inhibitory cytokine-1，MIC-1）是转化生长因子β超家族的一个成员。MIC-1转录上调与巨噬细胞活化相关，这一观察结果导致了其克隆。北方印迹表明MIC-1也存在于人胎盘中。一个敏感的夹心酶联免疫吸附试验定量MIC-1的开发和使用，以检查这种细胞因子在怀孕中的作用。孕妇血清中存在高水平的MIC-1。随着妊娠的进展，这一水平大幅上升。MIC-1也可以在羊水和胎盘提取物中大量检测到。此外，发现BeWo胎盘滋养层细胞系组成型表达MIC-1转录物并分泌大量MIC-1。这些发现表明，胎盘滋养层细胞是母体血清和羊水中MIC-1的主要来源。我们认为MIC-1可能通过抑制母体来源的促炎细胞因子在子宫内的产生来促进胎儿存活。
Macrophage inhibitory cytokine-1 (MIC-1) is a recently described divergent member of the transforming growth factor-beta superfamily. MIC-1 transcription up-regulation is associated with macrophage activation, and this observation led to its cloning. Northern blots indicate that MIC-1 is also present in human placenta. A sensitive sandwich enzyme-linked immunosorbent assay for the quantification of MIC-1 was developed and used to examine the role of this cytokine in pregnancy. High levels of MIC-1 are present in the sera of pregnant women. The level rises substantially with progress of gestation. MIC-1 can also be detected, in large amounts, in amniotic fluid and placental extracts. In addition, the BeWo placental trophoblastic cell line was found to constitutively express the MIC-1 transcript and secrete large amounts of MIC-1. These findings suggest that the placental trophoblast is a major source of the MIC-1 present in maternal serum and amniotic fluid. We suggest that MIC-1 may promote fetal survival by suppressing the production of maternally derived proinflammatory cytokines within the uterus.