Disrupted Genome Methylation in Response to High Temperature Has Distinct Affects on Microspore Abortion and Anther Indehiscence
Disrupted Genome Methylation in Response to High Temperature Has Distinct Affects on Microspore Abortion and Anther Indehiscence
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DOI：
10.1105/tpc.18.00074
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发表时间：
2018-07-01
期刊：
影响因子：
11.6
通讯作者：
Zhang, Xianlong
中科院分区：
文献类型：
--
作者：
Ma, Yizan;Min, Ling;Zhang, Xianlong
高温（HT）胁迫诱导雄性不育，导致作物产量降低。DNA甲基化调控着植物发育和胁迫反应的一系列过程，但其在高温下雄性不育中的作用尚不清楚。本文通过全基因组亚硫酸氢盐测序，研究高温和常温条件下棉花花药DNA甲基化水平，探讨高温下DNA甲基化对雄性育性的调控作用。在HT敏感系中检测到DNA甲基化的整体破坏，特别是CHH甲基化（其中H = A、C或T）。24-核苷酸小干扰RNA水平的变化与DNA甲基化水平显著相关。实验抑制DNA甲基化导致花粉不育的HT敏感线在NT条件下，但不影响花药壁的正常开裂。进一步的转录组分析表明，高温处理显著调节了花药中糖和活性氧代谢途径基因的表达，但生长素的合成和信号转导途径仅发生轻微改变，表明高温通过破坏DNA甲基化来干扰糖和活性氧代谢，导致小孢子不育。本研究为利用表观遗传技术培育耐HT品种开辟了一条途径。
High-temperature (HT) stress induces male sterility, leading to yield reductions in crops. DNA methylation regulates a range of processes involved in plant development and stress responses, but its role in male sterility under HT remains unknown. Here, we investigated DNA methylation levels in cotton (Gossypium hirsutum) anthers under HT and normal temperature (NT) conditions by performing whole-genome bisulfite sequencing to investigate the regulatory roles of DNA methylation in male fertility under HT. Global disruption of DNA methylation, especially CHH methylation (where H = A, C, or T), was detected in an HT-sensitive line. Changes in the levels of 24-nucleotide small-interfering RNAs were significantly associated with DNA methylation levels. Experimental suppression of DNA methylation led to pollen sterility in the HT-sensitive line under NT conditions but did not affect the normal dehiscence of anther walls. Further transcriptome analysis showed that the expression of genes in sugar and reactive oxygen species (ROS) metabolic pathways were significantly modulated in anthers under HT, but auxin biosynthesis and signaling pathways were only slightly altered, indicating that HT disturbs sugar and ROS metabolism via disrupting DNA methylation, leading to microspore sterility. This study opens up a pathway for creating HT-tolerant cultivars using epigenetic techniques.