Mutagenicity testing of organic extracts of diesel exhaust particles after spiking with polycyclic aromatic hydrocarbons (PAH)
Mutagenicity testing of organic extracts of diesel exhaust particles after spiking with polycyclic aromatic hydrocarbons (PAH)
复制标题
DOI：
10.1007/s002040050555
复制
发表时间：
1998-10-01
影响因子：
6.1
通讯作者：
Eide, I
中科院分区：
文献类型：
--
作者：
Bostrom, E;Engen, S;Eide, I
在本研究中，使用加标作为评价混合物中单个化合物致突变性的策略。研究了柴油机尾气颗粒物（DEP）有机提取物中多环芳烃（PAH）的致突变性。用二氯甲烷（DCM）萃取颗粒。用二甲基亚砜（DMSO）代替DCM后，向提取物中加入四种单独的PAH：苯并（a）芘、苯并（a）蒽、芘和荧蒽。将PAH单独和以各种组合添加到提取物中，以确定每个变量的影响，并确定单个PAH之间以及PAH与提取物之间可能的相互作用。该研究设计为五个变量（DEP浸提液和四种PAH）的部分析因实验，得到16种（而不是32种）混合物加上一式三份中心点和背景，即总共20种。在本工作中使用的部分析因设计支持一个模型与线性和交互作用的条款。在存在大鼠肝外源性酶（S9混合物）的情况下，使用4种鼠伤寒沙门氏菌菌株，在艾姆斯试验中检测混合物的致突变性。使用潜在结构预测（PLS）来量化每种化合物的致突变性和可能的相互作用。在含10% S9混合物的艾姆斯试验中，四种单独PAH和DEP浸提液具有相加作用。
In the present study, spiking was used as a strategy to evaluate the mutagenicity of individual compounds in a mixture. Mutagenicity of individual polycyclic aromatic hydrocarbons (PAH) was evaluated in an organic extract of diesel exhaust particles (DEP). The particles were extracted with dichloromethane (DCM). After replacing DCM with dimethylsulphoxide (DMSO), the extract was spiked with four individual PAH: benzo(a)pyrene, benzo(a)anthracene, pyrene and fluoroanthene. The PAH were added separately and in various combinations to the extract to determine the effects of each variable and to identify possible interactions between the individual PAH and between the PAH and the extract. The study was designed as a fractional factorial experiment with the five variables (the DEP extract and the four PAH), giving 16 (instead of 32) mixtures plus a triplicate centrepoint and background, i.e. a total of 20. The fractionated factorial design used in the present work supports a model with linear and interaction terms. The mixtures were tested for mutagenicity in the Ames assay using four strains of Salmonella typhimurium in the presence of rat liver xenobiotic enzymes (S9-mix). Projections to Latent Structures (PLS) was used to quantify the mutagenicity of each compound and possible interactions. The four individual PAH and the DEP extract acted additively in the Ames test with 10% S9-mix.