Association of I-J determinants with lipomodulin/macrocortin.
Association of I-J determinants with lipomodulin/macrocortin.
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I-J 决定因素与脂调节蛋白/大皮质素的关联。
DOI：
10.1073/pnas.83.1.160
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发表时间：
1986
影响因子：
11.1
通讯作者：
Ishizaka,K
中科院分区：
文献类型：
--
作者：
Jardieu,P;Akasaki,M;Ishizaka,K
用糖皮质激素刺激小鼠脾淋巴细胞和腹膜贴壁细胞，导致形成可溶性因子，即糖基化抑制因子 (GIF)，该因子在生物合成过程中抑制 N-连接寡糖与 IgE 结合因子的组装。 GIF的主要细胞来源分别是Lyt 2+ I-J+ T细胞和巨噬细胞。来自 T 细胞的 GIF 分子大小为 15 kDa，而来自巨噬细胞的 GIF 由 40 kDa 和 15 kDa 分子组成。来自两种细胞来源的 GIF 与抗脂调节蛋白的单克隆抗体结合，并在去磷酸化后发挥磷脂酶抑制活性。鉴于之前的报道称糖皮质激素诱导磷脂酶抑制蛋白（即脂调节蛋白/大皮质素）的形成，来自 T 细胞和巨噬细胞的 GIF 似乎是脂调节蛋白的磷酸化衍生物。从 C57BL/6 小鼠脾淋巴细胞和巨噬细胞释放的 GIF 与抗 I-Jb 抗体结合，但不与抗 I-Jk 或抗 I-Js 抗体结合。在用糖皮质激素刺激后，CBA和B10.A(5R)淋巴细胞释放GIF，该GIF与抗I-Jk抗体结合但不与抗I-Jb抗体结合，而来自B10.A(3R)淋巴细胞的相同因子与抗I-Jb抗体结合。结果表明 I-J 决定簇与来自 T 细胞和巨噬细胞的脂调节蛋白/大皮质素相关。
Stimulation with glucocorticoids of mouse splenic lymphocytes and peritoneal adherent cells resulted in the formation of a soluble factor--i.e., glycosylation inhibiting factor (GIF)--that inhibits the assembly of N-linked oligosaccharide(s) to IgE-binding factors during their biosynthesis. The major cell sources of GIF are Lyt 2+ I-J+ T cells and macrophages, respectively. The molecular size of GIF from T cells was 15 kDa, while GIF from macrophages consisted of 40- and 15-kDa molecules. GIF from both cell sources bound to monoclonal antibody against lipomodulin and exerted phospholipase inhibitory activity upon dephosphorylation. In view of previous reports that glucocorticoids induce the formation of phospholipase inhibitory protein--i.e., lipomodulin/macrocortin--GIF from T cells and macrophages appear to be phosphorylated derivatives of lipomodulin. GIF released from splenic lymphocytes and macrophages of C57BL/6 mice bound to anti-I-Jb antibodies but not to either anti-I-Jk or anti-I-Js antibodies. Upon stimulation with glucocorticoids, CBA and B10.A(5R) lymphocytes released GIF that bound to anti-I-Jk antibodies but not to anti-I-Jb antibodies, while the same factor from B10.A(3R) lymphocytes bound to anti-I-Jb antibodies. The results indicate that I-J determinants are associated with lipomodulin/macrocortin from T cells and macrophages.