PROSTATE ANTIGEN - A NEW POTENTIAL MARKER FOR PROSTATIC-CANCER
PROSTATE ANTIGEN - A NEW POTENTIAL MARKER FOR PROSTATIC-CANCER
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DOI：
10.1002/pros.2990020109
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发表时间：
1981-01-01
期刊：
影响因子：
2.8
通讯作者：
CHU, TM
中科院分区：
文献类型：
--
作者：
WANG, MC;PAPSIDORE, LD;CHU, TM
通过免疫学程序在前列腺组织（正常、良性肥大和癌性）和精浆以及前列腺癌患者和携带人前列腺肿瘤的裸鼠血清中鉴定出不同于酸性磷酸酶的前列腺特异性抗原。免疫过氧化物酶染色显示该抗原仅限于前列腺导管上皮细胞。从前列腺组织和精浆中纯化前列腺抗原，其分子量为33，000 - 34，000，无亚基组分。纯化抗原的等电点约为6.9，但也观察到几种具有不同等电点的未纯化异构体。血清载前列腺抗原显示分子量为90，000 - 100，000，但在十二烷基硫酸钠存在下显示分子量为36，000。开发了一种能够检测每毫升血液中0.1 ng抗原的夹心型过氧化物酶联免疫吸附试验。用这种技术，发现与正常男性相比，前列腺癌患者的血清抗原水平增加。前列腺特异性抗原可以是检测前列腺癌的有用标志物。
A prostate‐specific antigen, distinct from acid phosphatase, was identified by immunologic procedures in prostate tissues (normal, benign hypertrophic, and cancerous) and seminal plasma, as well as in sera of patients with prostatic cancer and of nude mice bearing human prostatic tumor. This antigen was shown by immunoperoxidase staining to be confined to epithelial cells comprising the prostatic ductal elements. Prostate antigen was purified from prostatic tissue and seminal plasma, and it was shown to have a molecular weight of 33,000‐34,000 with no subunit component. The isolectric point of purified antigen was around 6.9, though several unpurified isomers with different isoelec‐tric points also were observed. Serum‐borne prostate antigen showed a molecular weight of 90,000‐100,000, but it exhibited a molecular weight of 36,000 in the presence of sodium dodecyl sulfate. A sandwich‐type, peroxidase‐linked immunosorbent assay capable of detecting 0.1ng of the antigen per milliliter of blood was developed. With this technique, serum level of the antigen was found to increase in patients with prostatic cancer as compared with normal males. The prostate‐specific antigen can be a useful marker for detection of prostatic cancer.