QTL analysis of yield-related traits and their association with functional markers in Brassica napus L.
QTL analysis of yield-related traits and their association with functional markers in Brassica napus L.
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DOI：
10.1071/ar06350
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发表时间：
2007-01-01
影响因子：
--
通讯作者：
Ma, Chaozhi
中科院分区：
文献类型：
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作者：
Li, Yuanyuan;Shen, Jinxiong;Ma, Chaozhi
产量是甘蓝型油菜育种计划中最重要的性状之一。基于遗传图谱的产量相关性状的数量性状基因座（QTL）将有助于育种者开发高产品种。在本研究中，在 SI-1300 x Eagle 杂交产生的由 184 个个体组成的 F-2 群体中构建了甘蓝型油菜的遗传连锁图谱，其中包含 142 个序列相关扩增多态性 (SRAP) 标记、163 个功能标记、160 个简单序列重复 (SSR) 标记和 117 个扩增片段长度多态性 (AFLP) 标记。该地图覆盖 2054.51 cM，平均标记间隔为 3.53 cM。随后，在武汉和荆门检测到12个产量相关性状的QTL。总共鉴定出 133 个 QTL，其中 2 个地点有 14 个一致的 QTL。发现 20 个连锁群 (LG) 中的 15 个具有所调查的 12 个性状的 QTL，并且大多数 QTL 是聚集的，特别是在 LG N2 和 N7 上，其中多个性状鉴定出相似的 QTL 位置。每株产量 (YP) 的 10 个 QTL 中的 8 个也与每株长角果种子数 (SS)、每株长角果数 (SP) 和/或 1000 粒种子重量 (SW) 相关。此外，涉及39个表达序列标签（EST）的45个功能标记与12个性状的QTL连锁。目前的结果可以为甘蓝型油菜农艺性状的进一步分子解剖提供有价值的基础，并且与QTL相关的标记可以为标记辅助选择提供有前景的可能标记。
Yield is one of the most important traits in Brassica napus breeding programs. Quantitative trait loci (QTLs) for yield-related traits based on genetic mapping would help breeders to develop high-yield cultivars. In this study, a genetic linkage map of B. napus, containing 142 sequence-related amplified polymorphism (SRAP) markers, 163 functional markers, 160 simple sequence repeat (SSR) markers, and 117 amplified fragment length polymorphism (AFLP) markers, was constructed in an F-2 population of 184 individuals resulting from the cross SI-1300 x Eagle. This map covered 2054.51 cM with an average marker interval of 3.53 cM. Subsequently, QTLs were detected for 12 yield-related traits in Wuhan and Jingmen. In total, 133 QTLs were identified, including 14 consistent ones across the 2 locations. Fifteen of 20 linkage groups ( LGs) were found to have QTLs for the 12 traits investigated, and most of the QTLs were clustered, especially on LGs N2 and N7, where similar QTL positions were identified for multiple traits. Eight of 10 QTLs for yield per plant (YP) were also associated with number of seeds per silique ( SS), number of siliques per plant (SP), and/or 1000-seed weight ( SW). In addition, 45 functional markers involved in 39 expressed sequence tags ( ESTs) were linked to the QTLs of 12 traits. The present results may serve as a valuable basis for further molecular dissection of agronomic traits in B. napus, and the markers related to QTLs may offer promising possible makers for marker assisted selection.