Mre11 dimers coordinate DNA end bridging and nuclease processing in double-strand-break repair.
Mre11 dimers coordinate DNA end bridging and nuclease processing in double-strand-break repair.
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Mre11 二聚体在双链断裂修复中协调 DNA 末端桥接和核酸酶加工。
DOI：
10.1016/j.cell.2008.08.017
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发表时间：
2008-10-03
期刊：
影响因子：
64.5
通讯作者：
Tainer JA
中科院分区：
文献类型：
--
作者：
Williams RS;Moncalian G;Williams JS;Yamada Y;Limbo O;Shin DS;Groocock LM;Cahill D;Hitomi C;Guenther G;Moiani D;Carney JP;Russell P;Tainer JA
Mre11 forms the core of the multifunctional Mre11-Rad50-Nbs1 (MRN) complex that detects DNA double-strand breaks (DSBs), activates the ATM checkpoint kinase, and initiates homologous recombination (HR) repair of DSBs. To de?ne the roles of Mre11 in both DNA bridging and nucleolytic processing during initiation of DSB repair, we combined small-angle X-ray scattering (SAXS) and crystal structures of Pyrococcus furiosus Mre11 dimers bound to DNA with mutational analyses of ?ssion yeast Mre11. The Mre11 dimer adopts a four-lobed U-shaped structure that is critical for proper MRN complex assembly and for binding and aligning DNA ends. Further, mutations blocking Mre11 endonuclease activity impair cell survival after DSB induction without compromising MRN complex assembly or Mre11-dependant recruitment of Ctp1, an HR factor, to DSBs. These results show how Mre11 dimerization and nuclease activities initiate repair of DSBs and collapsed replication forks, as well as provide a molecular foundation for understanding cancer-causing Mre11 mutations in ataxia telangiectasia-like disorder (ATLD).
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影响因子：
64.5
作者：
Buis J;Wu Y;Deng Y;Leddon J;Westfield G;Eckersdorff M;Sekiguchi JM;Chang S;Ferguson DO
通讯作者：
Ferguson DO
影响因子：
16
作者：
Lengsfeld, Bettina M.;Rattray, Alison J.;Paull, Tanya T.
通讯作者：
Paull, Tanya T.
影响因子：
5.3
作者：
Chahwan, C;Nakamura, TM;Rhind, N
通讯作者：
Rhind, N
影响因子：
64.8
作者：
Moreno-Herrero, F;de Jager, M;Dekker, C
通讯作者：
Dekker, C
DOI：
10.1073/pnas.110144297
发表时间：
2000-06-06
影响因子：
11.1
作者：
Paull, TT;Gellert, M
通讯作者：
Gellert, M