Collaborative studies of zeta-potential measurements and electrophoretic measurements using reference sample
Collaborative studies of zeta-potential measurements and electrophoretic measurements using reference sample
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DOI：
10.1016/s0927-7757(97)00280-x
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发表时间：
1998-09-30
影响因子：
5.2
通讯作者：
Uchiyama, K
中科院分区：
文献类型：
--
作者：
Furusawa, K;Uchiyama, K
同时测量两种标准乳胶悬浮液的 zeta 电位，以评估每种测量技术的可靠性并找到改进方法。此外，在各种实验条件下合成了在水性介质中空间稳定且没有任何静电效应的参比颗粒分散体，并使用参比分散体作为标准测量了zeta电位。很明显，在高于LCST的温度下，在表面电荷密度较低的乳胶颗粒上形成具有较低临界溶液温度（LCST）的羟丙基纤维素（HPC）致密吸附层，起到完全屏蔽裸露颗粒表面电荷产生的静电效应的作用。这种具有零 zeta 电位的参考粒子使我们能够通过减去相同水平的参考样品的迁移率来确定未知样品在电泳池中二分之一深度处的电泳迁移率。此外，细胞壁的 zeta 电位可以根据参考样品的迁移率轻松确定，因为参考样品的表观速度分布表明了细胞中的液体流速。 (C) 1998 Elsevier Science B.V. 保留所有权利。
Simultaneous measurements of zeta-potential for two standard latex suspensions were carried out so as to assess the reliability of each of these measurement techniques and find means for their improvement. Furthermore, syntheses of a reference particle dispersion stabilized sterically in an aqueous medium without any electrostatic effects and measurements of zeta-potential using the reference dispersion as a standard were performed under various experimental conditions. It became apparent that the dense adsorption layer of hydroxypropylcellulose (HPC), with a lower critical solution temperature (LCST), formed on latex particles with a low surface charge density at temperatures higher than the LCST, plays a role in completely shielding the electrostatic effect arising from the surface charge on the bare particles. Such reference particles with zero zeta-potential allow us to determine the.electrophoretic mobility of unknown samples at the one-half depth in the electrophoretic cell by subtracting the mobility of the reference sample at the same level. Furthermore, the zeta-potential of the cell wall can be easily determined from the mobility of the reference sample, because the apparent velocity profile of the reference sample indicates the liquid flow velocity in the cell. (C) 1998 Elsevier Science B.V. All rights reserved.