Evaluating Phospholipid-Functionalized Gold Nanorods for In Vivo Applications.
Evaluating Phospholipid-Functionalized Gold Nanorods for In Vivo Applications.
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DOI：
10.1002/smll.202006797
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发表时间：
2021-03
期刊：
影响因子：
13.3
通讯作者：
Lucien Roach;M. E. Booth;N. Ingram;D. Paterson;Damien V. B. Batchelor;Samuel C T Moorcroft;R. Bushby;K. Critchley;P. Coletta;S. Evans
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文献类型：
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作者：
Lucien Roach;M. E. Booth;N. Ingram;D. Paterson;Damien V. B. Batchelor;Samuel C T Moorcroft;R. Bushby;K. Critchley;P. Coletta;S. Evans
金纳米棒（AuNRs）由于其在广泛的生物医学应用中的潜力而引起了极大的关注。然而，它们的生产通常需要使用相对有毒的阳离子表面活性剂十六烷基三甲基溴化铵（CTAB），导致对用于体内应用的解毒方案的持续需求。在这项研究中，提出了一个强大的和简便的协议，从表面的金核受体使用磷脂的CTAB的位移。置换后，CTAB不能通过NMR光谱、表面增强拉曼光谱或使用pH依赖性拉曼电位测量来检测。磷脂官能化的AuNR与CTAB和EDTA官能化的AuNR相比表现出上级的稳定性和生物相容性（IC 50> 200 μg mL-1），并且在体内耐受良好。此外，它们具有高的近红外（NIR）吸收率，并在NIR照射下产生大量的热，因此这种颗粒非常适合于等离子体医学应用。
Gold nanorods (AuNRs) have attracted a great deal of attention due to their potential for use in a wide range of biomedical applications. However, their production typically requires the use of the relatively toxic cationic surfactant cetyltrimethylammonium bromide (CTAB) leading to continued demand for protocols to detoxify them for in vivo applications. In this study, a robust and facile protocol for the displacement of CTAB from the surface of AuNRs using phospholipids is presented. After the displacement, CTAB is not detectable by NMR spectroscopy, surface-enhanced Raman spectroscopy, or using pH-dependent ζ-potential measurements. The phospholipid functionalized AuNRs demonstrated superior stability and biocompatibility (IC50 > 200 µg mL-1 ) compared to both CTAB and polyelectrolyte functionalized AuNRs and are well tolerated in vivo. Furthermore, they have high near-infrared (NIR) absorbance and produce large amounts of heat under NIR illumination, hence such particles are well suited for plasmonic medical applications.