Cannabinoid 1 receptors are expressed by nerve growth factor- and glial cell-derived neurotrophic factor-responsive primary sensory neurones
Cannabinoid 1 receptors are expressed by nerve growth factor- and glial cell-derived neurotrophic factor-responsive primary sensory neurones
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DOI：
10.1016/s0306-4522(01)00601-7
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发表时间：
2002-01-01
期刊：
影响因子：
3.3
通讯作者：
Nagy, I
中科院分区：
文献类型：
--
作者：
Ahluwalia, J;Urban, L;Nagy, I
研究了大麻素1 （cannabinoid 1, CB1）受体在培养的成年大鼠初级感觉神经元不同伤害性亚群和非伤害性亚群中的表达及其调控。采用单纯板连分离素B4 （IB4）结合和降钙素基因相关肽（CGRP）免疫染色区分神经胶质细胞源性神经营养因子（GDNF）和神经生长因子（NGF）反应性伤害感受神经元和非伤害感受初级感觉神经元，同时采用CB1受体特异性抗体研究CB1受体蛋白的表达。在两种神经营养因子（各50 ng/ml）存在下培养1天，约一半（47 +/- 3.2%）的初级感觉神经元显示CB1受体样免疫染色，而21.8 +/- 3.3%和32.7 +/- 5.6%的神经元分别显示cgrp样免疫阳性和IB4结合。一部分CB1受体样免疫阳性神经元进行了CGRP（31.7 +/- 5%）和IB4（48.2% +/- 7.5%）的免疫染色，CGRP和IB4结合的共表达极小（1%）。约五分之一的CB1受体样免疫阳性神经元未显示cgrp样免疫染色或IB4结合。为了了解cbi受体在伤害性初级感觉神经元中的表达是否受GDNF或NGF的调节，我们在存在或不存在神经营养因子的情况下培养7天。采用香草样受体1 （VR1）免疫染色作为对照标志物监测神经营养因子的作用。在这两种因子（各50 ng/ml）存在的培养中，51 +/- 2.6%和42.4 +/- 1.2%的细胞分别显示CB1受体样和vr1样免疫染色。在没有任何一种神经营养因子的培养7天中，vr1样免疫阳性细胞的相对数量减少到13.4 +/- 2.7%，而CB1受体样免疫阳性神经元的相对数量保持不变（50.6 +/- 1.1%）。我们的数据表明，CB1受体在初级感觉神经元的所有三个主要亚群中都有表达，并且CB1受体的表达不受NGF或GDNF的调节。(c) 2002年ibro。Elsevier Science Ltd.出版。版权所有。
Expression of the cannabinoid 1 (CB1) receptor and its regulation were studied in the different nociceptive and non-nociceptive sub-populations of cultured primary sensory neurones of adult rats. Bandairaea simplicifolia isolectin B4 (IB4) binding and calcitonin gene-related peptide (CGRP) immunostaining were used to distinguish between the glial cell-derived neurotrophic factor (GDNF)- and nerve growth factor (NGF)-responsive nociceptive and the non-nociceptive primary sensory neurones while a specific CB1 receptor antibody was used to study the expression of the CB1 receptor protein. About half of the total number of primary sensory neurones (47 +/- 3.2%) cultured for 1 day in the presence of both neurotrophic factors (50 ng/ml each) showed CB1 receptor-like immunostaining, whereas 21.8 +/- 3.3% and 32.7 +/- 5.6% of the neurones showed CGRP-like immunopositivity and IB4 binding, respectively. A proportion of the CB1 receptor-like immunopositive neurones was immunostained for CGRP (31.7 +/- 5%) and IB4 (48.2% +/- 7.5), with a minimal (1%) co-expression of CGRP and IB4 binding. About a fifth of the CB1 receptor-like immunopositive neurones did not show either CGRP-like immunostaining or IB4 binding.To find out whether CB I receptor expression in nociceptive primary sensory neurones is regulated by GDNF or NGF, cultures were grown in the presence or absence of the neurotrophic factors for 7 days. Vanilloid receptor 1 (VR1) immunostaining was used as a control marker to monitor the effect of the neurotrophins. In cultures maintained in the presence of both factors (50 ng/ml each) 51 +/- 2.6% and 42.4 +/- 1.2% of the cells showed CB1 receptor-like and VR1-like immunostaining, respectively, In cultures grown for 7 days in the absence of either of the neurotrophic factors the relative number of VR1-like immunopositive cells decreased to 13.4 +/- 2.7%, whereas the relative number of CB1 receptor-like immunopositive neurones was unchanged (50.6 +/- 1.1%).Our data suggest that the CB1 receptor is expressed in all of the three major sub-populations of primary sensory neurones and that the CB1 receptor expression is not regulated by either NGF or GDNF. (C) 2002 IBRO. Published by Elsevier Science Ltd. All rights reserved.