CRITICAL STRUCTURAL ELEMENTS OF THE VP16 TRANSCRIPTIONAL ACTIVATION DOMAIN
CRITICAL STRUCTURAL ELEMENTS OF THE VP16 TRANSCRIPTIONAL ACTIVATION DOMAIN
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DOI：
10.1126/science.1846049
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发表时间：
1991-01-04
期刊：
影响因子：
56.9
通讯作者：
TRIEZENBERG, SJ
中科院分区：
文献类型：
--
作者：
CRESS, WD;TRIEZENBERG, SJ
单纯疱疹病毒1型的病毒体蛋白16（VP16）含有酸性转录激活结构域。 该结构域中的错义突变提供了对其功能至关重要的结构元件的见解。 净负电荷有助于，但不足以，转录激活VP16。 一个假定的两亲性α螺旋似乎不是激活域的重要结构组成部分。 442位的苯丙氨酸残基对突变非常敏感。 几类转录激活剂含有疏水氨基酸，其排列模式类似于VP16。 因此，VP16和其他蛋白质的转录激活机制可能涉及与靶分子的离子和特异性疏水相互作用。
Virion protein 16 (VP16) of herpes simplex virus type 1 contains an acidic transcriptional activation domain. Missense mutations within this domain have provided insights into the structural elements critical for its function. Net negative charge contributed to, but was not sufficient for, transcriptional activation by VP16. A putative amphipathic alpha helix did not appear to be an important structural component of the activation domain. A phenylalanine residue at position 442 was exquisitely sensitive to mutation. Transcriptional activators of several classes contain hydrophobic amino acids arranged in patterns resembling that of VP16. Therefore, the mechanism of transcriptional activation by VP16 and other proteins may involve both ionic and specific hydrophobic interactions with target molecules.