Epitope Analysis and Efficacy Evaluation of Phosphatase 2C (PP2C) DNA Vaccine Against Toxoplasma gondii Infection.
Epitope Analysis and Efficacy Evaluation of Phosphatase 2C (PP2C) DNA Vaccine Against Toxoplasma gondii Infection.
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DOI：
10.1645/18-210
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发表时间：
2020-08
期刊：
影响因子：
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通讯作者：
P. Song;S. Yao;Y. Yao;Jian Zhou;Q. F. Li;Y. H. Cao;Shen-yi He
中科院分区：
文献类型：
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作者：
P. Song;S. Yao;Y. Yao;Jian Zhou;Q. F. Li;Y. H. Cao;Shen-yi He
弓形虫感染几乎所有温血动物，并对包括人类在内的多种动物的健康造成负面影响。蛋白磷酸酶2C(PP2C)是弓形虫的一种蛋白质，在宿主细胞入侵过程中由杆状细胞器分泌。然而，关于这种蛋白是否能诱导对弓形虫的保护性免疫，人们知之甚少。在这项研究中，PP2C的生物信息学分析揭示了一些在抗弓形虫病治疗和疫苗研究方面的有用信息。此外，还扩增了PP2C基因，并成功构建了表达PP2C的真核表达载体pEGFP-PP2C。最后，将构建的pEGFP-PP2C注射到小鼠体内，评价其是否具有免疫保护作用。与对照组比较，pEGFP-PP2C免疫小鼠可诱导产生抗弓形虫感染的特异性抗体和细胞因子。与对照组相比，pEGFP-PP2C免疫小鼠的存活时间明显延长。基于pEGFP-PP2C诱导弓形虫特异性免疫应答的能力，我们认为PP2C可作为弓形虫病的潜在候选疫苗。
Toxoplasma gondii infects almost all warm-blooded animals and negatively affects the health of a wide range of these animals, including humans. Protein phosphatase 2C (PP2C) is a T. gondii protein secreted by rhoptry organelles during host cell invasion. However, very little is known about whether this protein can induce protective immunity against T. gondii. In this study, bioinformatics analysis of PP2C revealed some useful information in the context of anti-toxoplasmosis treatments and vaccine research. In addition, the PP2C gene was amplified, and a eukaryotic expression vector (pEGFP-PP2C) was successfully constructed to express PP2C. Finally, the constructed pEGFP-PP2C was injected into mice to evaluate whether it could induce immunoprotection. Compared with the control groups, we found that immunizations with the pEGFP-PP2C plasmid could elicit specific IgG antibodies and cytokines against T. gondii infection. The survival of mice immunized with the pEGFP-PP2C plasmid was significantly prolonged compared with that of the control group mice. Based on the ability of pEGFP-PP2C to induce specific immune responses against T. gondii, we propose that PP2C merits consideration as a potential vaccine candidate against toxoplasmosis.