SHIGELLA INFECTION INDUCES CELLULAR ACTIVATION OF T-CELLS AND B-CELLS AND DISTINCT SPECIES-RELATED CHANGES IN PERIPHERAL-BLOOD LYMPHOCYTE SUBSETS DURING THE COURSE OF THE DISEASE
SHIGELLA INFECTION INDUCES CELLULAR ACTIVATION OF T-CELLS AND B-CELLS AND DISTINCT SPECIES-RELATED CHANGES IN PERIPHERAL-BLOOD LYMPHOCYTE SUBSETS DURING THE COURSE OF THE DISEASE
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DOI：
10.1128/iai.63.8.2941-2949.1995
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发表时间：
1995-08-01
影响因子：
3.1
通讯作者：
CHRISTENSSON, B
中科院分区：
文献类型：
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作者：
ISLAM, D;BARDHAN, PK;CHRISTENSSON, B
使用三色流式细胞术对志贺氏菌病患者外周血淋巴细胞（T、B 和 NK 细胞）的免疫表型变化进行了表征。研究人员对 11 名志贺氏菌 1 感染成人患者 (SDIP)、11 名福氏志贺氏菌感染成人患者 (SFIP)、15 名来自孟加拉国的年龄和性别匹配的健康对照者 (C-B) 以及 15 名来自瑞典的健康志愿者 (V-S) 进行了研究。在SDIP和SFIP中，CD4(+)和CD8(+) T细胞中的CD45RO(+)细胞显着增加。我们发现了 T 细胞连续激活的证据，如 CD25 和 CD4(+) 细胞比例增加所示； CD8(+)细胞上的HLA DR和CD38，以及CD4(+)和CD8(+)细胞上的CD54。我们发现与感染志贺氏菌属相关的淋巴细胞激活和子集模式存在差异。因此，在 SFIP 中观察到 CD45 表达减少；在疾病期间这种下降进一步加剧。 NK细胞（CD56+细胞）和CD3-CD8+细胞占CD8+细胞总数的比例在SFIP中增加，但在SDIP中没有增加。 SDIP组CD3(+)CD8(+)CD57(+)T细胞亚群显着低于C-B组，表达激活标志物CD80和CD23的B淋巴细胞比例高于C-B组。与 C-B 相比，V-S 组 CD4(+) T 细胞比例显着增加，总 B 细胞、CD3(+) CD8(+) CD57(+) T 细胞亚群和 CD56(+) CD16(+) NK 细胞亚群比例显着下降。我们的结果表明，与 SFIP 相比，SDIP 引起了不同的子集变化和激活模式，并且激活程度与疾病严重程度相关。此外，在疾病过程中发现了常见的细胞激活顺序。 C-B 和 V-S 中看到的子集模式的差异可能与环境中传染原的水平或谱的差异有关。
Immunophenotypic changes in peripheral blood lymphocytes (T, B, and NK cells) in patients during shigellosis was characterized by using triple-color flow cytometry. Eleven Shigella dysenteriae 1-infected adult patients (SDIP), 11 Shigella flexneri-infected adult patients (SFIP), 15 age- and sex-matched healthy controls from Bangladesh (C-B), and 15 healthy volunteers from Sweden (V-S) were studied. In SDIP and SFIP, a significant increase in the CD45RO(+) cells in both CD4(+) and CD8(+) T cells was seen. We found evidence for sequential T-cell activation, as shown by increased proportions of CD25 and CD4(+) cells; HLA DR and CD38 on CD8(+) cells, and CD54 on CD4(+) and CD8(+) cells. We found differences in the lymphocyte activation and subset patterns related to the infecting Shigella species. Thus, a decrease in CD45 expression was seen in SFIP; this decrease progressed further during the disease. The proportions of NK cells (CD56(+) cells) and CD3(-) CD8+ cells out of the total CD8(+) cells were increased in SFIP but not in SDIP. The CD3(+) CD8(+) CD57(+) T-cell subset was significantly lower in SDIP than in C-B, The proportion of B-lymphocyte-expressing activation markers CD80 and CD23 was higher in patients than in C-B. There was a significant increase in the proportion of CD4(+) T cells and a significant decrease in the percentages of total B cells, the CD3(+) CD8(+) CD57(+) T-cell subset, and the CD56(+) CD16(+) NK-cell subset for V-S compared with C-B. Our results indicate that distinct subset changes and activation patterns are elicited in SDIP compared with SFIP and also that the degree of activation is related to disease severity. In addition, a common sequence of cell activation was seen during the disease course. The difference in the subset patterns seen in C-B and V-S may be related to differences in the levels or spectra of infectious agents in the environment.