INCORPORATION OF TRITIATED THYMIDINE BY EUCARYOTIC MICROALGAE 1
INCORPORATION OF TRITIATED THYMIDINE BY EUCARYOTIC MICROALGAE 1
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真核微藻 1 掺入氚三标记胸苷
DOI：
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发表时间：
1986
期刊：
影响因子：
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通讯作者：
R. Rivkin
中科院分区：
文献类型：
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作者：
R. Rivkin
测量了海洋硅藻和甲藻的无菌实验室培养物对氚化胸苷（3 H-TdR）的吸收和掺入。在胞质分裂之前的2至6小时期间，而不是在细胞周期的其他时间期间，所有四种藻类都将3 H-TdR掺入核酸中。从DNA中回收了90 - 95%的掺入（冷三氯乙酸不溶性）核酸中的3 H标记。3 H-TdR的掺入似乎准确地指示了DNA合成的时间。真核藻类在长期（24小时）孵育期间掺入3 H-TdR通常不排除使用3 H-TdR摄取来估计短期孵育期间的细菌产量和生长。
The uptake and incorporation of tritiated thymidine (3H‐TdR) by axenic laboratory cultures of marine diatoms and dinoflagellates was measured. 3H‐TdR was incorporated into nucleic acids by all four algae examined during a two to six hour period prior to cytokinesis and not during other times of the cell cycle. Between 90‐95% of the 3H label incorporated into (cold trichloroacetic acid insoluble) nucleic acids was recovered from DNA. Incorporation of 3H‐TdR appears to accurately indicate the timing of DNA synthesis. The incorporation of 3H‐TdR by eucaryotic algae during long term (24 h) incubations does not generally preclude using 3H‐TdR uptake to estimate bacterial production and growth during short term incubations.