The differential expression patterns of messenger RNAs encoding Nogo-A and Nogo-receptor in the rat central nervous system
The differential expression patterns of messenger RNAs encoding Nogo-A and Nogo-receptor in the rat central nervous system
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DOI：
10.1016/j.molbrainres.2004.10.004
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发表时间：
2005-01-05
期刊：
影响因子：
--
通讯作者：
Sato, K
中科院分区：
文献类型：
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作者：
Hasegawa, T;Ohno, K;Sato, K
Nogo-A 和 Nogo 受体被认为在控制轴突再生和神经元可塑性方面发挥着关键作用。我们使用原位杂交组织化学研究了成年大鼠中枢神经系统中 Nogo-A 和 Nogo 受体 mRNA 的总分布。 Nogo-A 在整个中枢神经系统的神经元和少突胶质细胞中大量表达。有趣的是，我们没有发现任何神经元缺乏Nogo-A mRNA表达，这表明Nogo-A mRNA在所有神经元中普遍表达。相比之下。 Nogo-R mRNA 表达非常有限。 Nogo-R mRNA在嗅球、海马、顶盖、部分杏仁核、大脑皮层、部分丘脑核、内侧缰核中表达，而在其他区域未检测到。有趣的是，我们没有在黑质、腹侧被盖区、蓝斑和中缝核的单胺能神经元中检测到 Nogo-R mRNA，这些神经元已知具有较高的再生能力。此外，虽然网状丘脑和小脑核中的神经元也显示出高再生能力，但在那里没有检测到 Nogo-R mRNA。这些数据表明，Nogo-A 和 Nogo-R mRNA 在中枢神经系统中存在差异表达，并表明给定神经元中缺乏 Nogo-R 表达可能是保持其高再生能力所必需的。 (C) 2004 Elsevier B.V. 保留所有权利。
Nogo-A and Nogo-receptor have been considered to play pivotal roles in controlling axonal regeneration and neuronal plasticity. We investigated the total distribution of Nogo-A and Nogo-receptor mRNAs in the adult rat central nervous system using in situ hybridization histochemistry. Nogo-A is abundantly expressed in both neurons and oligodendrocytes throughout the central nervous system. Interestingly, we could not find any neuron which lacks Nogo-A mRNA expression, indicating that Nogo-A mRNA is universally expressed in all neurons. In contrast. Nogo-R mRNA expression was very restricted. Nogo-R mRNA was expressed in the olfactory bulb, hippocampus, tentia tecta, some amygdala nuclei, cerebral cortex, some thalamic nuclei, medial habenular, whereas we could not detect it in the other regions. Interestingly, we did not detect Nogo-R mRNA in monoaminergic neurons, which are known to have high regenerative capacity, in the substantia nigra, ventral tegmental area, locus caeruleus, and raphe nuclei. In addition, although neurons in the reticular thalamus and cerebellar nuclei are also known to show high capacity for regeneration, Nogo-R mRNA was not detected there. These data indicate that Nogo-A and Nogo-R mRNAs were differentially expressed in the central nervous system, and suggest that the lack of Nogo-R expression in a given neuron might be necessary to keep its high regenerative capacity. (C) 2004 Elsevier B.V. All rights reserved.